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Expression of Bcl\u20102, pRB, p16<jats:sup>INK4A<\/jats:sup>, p53, c\u2010Myc, E\u2010cadherin, and EGFR was examined using tissue microarrays containing tumor specimens through immunohistochemistry. Three\u2010year DFS rates were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 65 consecutive patients were identified, of which 28 were excluded due to insufficient tissue (n\u2009=\u200922) and low biomarker quality (n\u2009=\u20096). Therefore, 37 patients with complete records were included. The included patients were significantly older and had a more advanced type of cordectomy than did the excluded patients (<jats:italic>P<\/jats:italic>\u2009=\u2009.015 and .009, respectively). According to the findings of univariate analysis, age, betel quid chewing, type of cordectomy, BCL\u20102 expression, and pRB expression significantly predicted 3\u2010year DFS. According to the findings of multivariate analysis, age (adjusted hazard ratio: 0.94, 95% CI: 0.88\u20101.00), betel quid chewing (adjusted hazard ratio: 5.07, 95% CI: 1.32\u201019.44), and pRB expression (adjusted hazard ratio: 0.02, 95% CI: 0.00\u20100.28) were independent predictors of 3\u2010year DFS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Low pRB expression is a potential biomarker for predicting disease relapse after primary TLM for early\u2010stage glottic SCC and may help to identify high\u2010risk patients who can subsequently undergo intensive management.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>4<\/jats:p><jats:p><jats:italic>Laryngoscope<\/jats:italic>, 129:E220\u2013E226, 2019<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27612","type":"journal-article","created":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T09:17:16Z","timestamp":1541063836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Low expression of pRB predicts disease relapse in early glottic cancer treated with transoral laser microsurgery"],"prefix":"10.1002","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8414-595X","authenticated-orcid":false,"given":"Li\u2010Ang","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8762-3635","authenticated-orcid":false,"given":"Tuan\u2010Jen","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck 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