{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:16:16Z","timestamp":1781100976954,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T00:00:00Z","timestamp":1545264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630024"],"award-info":[{"award-number":["81630024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570899"],"award-info":[{"award-number":["81570899"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81325006"],"award-info":[{"award-number":["81325006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470677"],"award-info":[{"award-number":["81470677"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670911"],"award-info":[{"award-number":["81670911"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700893"],"award-info":[{"award-number":["81700893"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Activation of mast cells associates with eosinophilic inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP). The disease\u2010specific mast cell\u2010triggering mechanisms apart from immunoglobulin E are poorly understood in CRSwNP. CD30L\/CD30 are members of the tumor necrosis factor\/receptor superfamily and display immune modulatory function on mast cells. The aim of this study was to explore the expression and function of CD30 and CD30L in CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The mRNA expression of CD30 and CD30L was analyzed by real\u2010time polymerase chain reaction. The cellular expression of CD30L was determined by immunofluorescence staining. The soluble CD30 levels in nasal tissues were detected by enzyme\u2010linked immunosorbent assay. HMC\u20101 cells, a human mast cell line, were cultured and stimulated with CD30.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with control tissues, CD30 mRNA expression levels were increased in eosinophilic polyps, and soluble CD30 protein levels were upregulated in both eosinophilic and noneosinophilic polyps with a greater increase in eosinophilic type. CD30 was expressed by T cells and B cells in nasal polyps. The CD30L mRNA expression levels and the number of CD30L<jats:sup>+<\/jats:sup> cells and CD30L<jats:sup>+<\/jats:sup>tryptase<jats:sup>+<\/jats:sup> mast cells were increased in eosinophilic polyps but not in noneosinophilic polyps as compared with control tissues. Mast cells accounted for 60% of CD30L<jats:sup>+<\/jats:sup> cells in eosinophilic polyps. CD30 induced HMC\u20101 cells to produce interleukin (IL)\u20104 and IL\u201013 without degranulation. Mast cells expressed IL\u20104 and IL\u201013 in eosinophilic polyps. The number of CD30L<jats:sup>+<\/jats:sup>tryptase<jats:sup>+<\/jats:sup> mast cells was positively correlated with the number of eosinophils and total inflammatory cells in eosinophilic polyps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD30\/CD30L\u2010mediated mast cell activation may promote the eosinophilic inflammation in CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 129:E110\u2013E117, 2019<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27658","type":"journal-article","created":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T11:11:41Z","timestamp":1545304301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Increased accumulation of CD30 ligand\u2010positive mast cells associates with eosinophilic inflammation in nasal polyps"],"prefix":"10.1002","volume":"129","author":[{"given":"Guan\u2010Ting","family":"Zhai","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Xian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of 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