{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:45:32Z","timestamp":1761709532109,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000297","name":"Royal College of Surgeons of England","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000828","name":"Royal Society of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000828","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["108768"],"award-info":[{"award-number":["108768"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Translational research into subglottic disease is restricted by the availability of primary human tissue originating from this subsite. Primary epithelial cells are also limited by their inability to survive beyond several divisions in culture outside of the body. Specific subglottic cell lines, useful for in vitro studies, have not yet been described. We therefore demonstrate what we believe to be the first immortalized subglottic epithelial cell line.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Subglottic tissue was derived from a single adult patient's neoplasia\u2010free human subglottic brushing specimen. Cells were immortalized using a lentiviral vector expressing simian virus 40 T antigen. Karyotyping was performed on the transformed cells using single nucleotide polymorphism array comparative genomic hybridization. Transformed cells were phenotypically characterized by light microscopy, immunohistochemistry, and electrophysiology studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The immortalized subglottic cell line (SG01) was able to divide successfully beyond 20 passages. Karyotyping demonstrated no significant genomic imbalance after immortalization. The cells demonstrated normal epithelial morphology and cytokeratin expression throughout. SG01 cells were also successfully cultured at air\u2013liquid interface (ALI). At ALI cells demonstrated cilia, mucus production, and relevant ion channel expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The novel SG01 subglottic epithelial cell line has been established. This cell line provides a unique resource for researchers to investigate subglottic diseases, such as subglottic stenosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 129:2640\u20132645, 2019<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27761","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T08:21:26Z","timestamp":1547022086000},"page":"2640-2645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Establishment of an immortalized human subglottic epithelial cell line"],"prefix":"10.1002","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-8406","authenticated-orcid":false,"given":"Jason","family":"Powell","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Freeman Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust  Newcastle upon Tyne United Kingdom"},{"name":"Institute of Cellular Medicine  Newcastle upon Tyne United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Verdon","sequence":"additional","affiliation":[{"name":"Institute for Cell and Molecular Biosciences  Newcastle upon Tyne United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet A.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Freeman Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust  Newcastle upon Tyne United Kingdom"},{"name":"Institute of Health and Society Newcastle University  Newcastle upon Tyne United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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