{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T11:19:43Z","timestamp":1773055183207,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010834","name":"American Laryngological Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["K23DC014747"],"award-info":[{"award-number":["K23DC014747"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC014728"],"award-info":[{"award-number":["R01DC014728"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["T32DC012280"],"award-info":[{"award-number":["T32DC012280"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>The larynx is a highly responsive organ exposed to mechanical, thermal, and chemical stimuli. Chemicals elicit responses both in intraepithelial nerve fibers and in specialized chemosensory cells, including scattered solitary cells as well as taste cells organized into taste buds. Activation of both chemosensory cells and taste buds in the larynx elicit cough, swallow, or apnea with exposure to sour or bitter substances, and even by water or sweet\u2010tasting chemicals. In an effort to begin understanding their function, we sought to compare the distribution, density, and types of chemosensory cells and chemoresponsive nerve fibers in laryngeal epithelium of humans and mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal and human laboratory analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using immunohistochemistry, we identified taste cells and polymodal nociceptive nerve fibers in the arytenoid area of the laryngeal epithelium of the following: 1) infants undergoing supraglottoplasty for laryngomalacia, and 2) a cadaveric specimen procured from a 34\u2010year\u2010old donor. We then compared these findings to both preweanling and mature mouse tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Arytenoid tissue from both human and mouse contained many taste buds containing type II taste cells\u2014bitter, sweet, or umami sensing\u2014which were innervated by nerve fibers expressing P2X3 type adenosine triphosphate receptors. Type III cells (acid responsive) were also present, but they were fewer in human tissue than in equivalent tissue from mice. In both species, the epithelium was densely innervated by free nerve endings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that from a standpoint of chemosensation, human and mouse larynges are biologically similar. This suggests that a murine model can be used effectively in laryngeal chemosensory research.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 130:423\u2013430, 2020<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27931","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T18:24:27Z","timestamp":1553538267000},"page":"423-430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Chemical receptors of the arytenoid: A comparison of human and mouse"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-1289","authenticated-orcid":false,"given":"Marie E.","family":"Jett\u00e9","sequence":"first","affiliation":[{"name":"Department of Otolaryngology in the School of Medicine at the University of Colorado Aurora Colorado U.S.A."},{"name":"Rocky Mountain Taste and Smell Center Aurora Colorado U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew S.","family":"Clary","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology in the School of Medicine at the University of Colorado Aurora Colorado U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Prager","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology in the School of Medicine at the University of Colorado Aurora Colorado U.S.A."},{"name":"Children's Hospital Colorado Aurora Colorado U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Finger","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Aurora Colorado U.S.A."},{"name":"Rocky Mountain Taste and Smell Center Aurora Colorado U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2009.411"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2007.01401.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2006.11.011"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000241157"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.53.2.455"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(75)90235-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00455-012-9417-x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-5998(98)70378-X"},{"key":"e_1_2_7_10_1","first-page":"801","article-title":"Otolaryngologic manifestations of gastroesophageal reflux","volume":"86","author":"Gaynor 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