{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T12:38:58Z","timestamp":1786797538967,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T00:00:00Z","timestamp":1557100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["RO1 DC013277"],"award-info":[{"award-number":["RO1 DC013277"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Development of novel vocal fold (VF) therapeutics is limited by a lack of standardized, meaningful outcomes. We hypothesize that automated microindentation\u2010based VF biomechanical property mapping matched to histology permits quantitative assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Ex vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twelve anesthetized New Zealand white rabbits underwent endoscopic right VF injury. Larynges were harvested\/bisected day 7, 30, or 60 (n = 4\/group), with four uninjured controls. Biomechanical measurements (normal force, structural stiffness, and displacement at 1.96 mN) were calculated using automated microindentation mapping (0.3 mm depth, 1.2 mm\/s, 2 mm spherical indenter) with a grid overlay (&gt;50 locations weighted toward VF edge, separated into 14 zones). Specimens were marked\/fixed\/sectioned, and slides matched to measurement points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the injury zone, normal force\/structural stiffness (mean, standard deviation [SD]\/mean, SD) increased from uninjured (2.2 mN, 0.64\/7.4 mN\/mm, 2.14) and day 7 (2.7 mN, 0.75\/9.0 mN\/mm, 2.49) to day 30 (4.3 mN, 2.11\/14.2 mN\/mm, 7.05) and decreased at 60 days (2.7 mN, 0.77\/9.1 mN\/mm, 2.58). VF displacement decreased from control (0.28 mm, 0.05) and day 7 (0.26 mm, 0.05) to day 30 (0.20 mm, 0.05), increasing at day 60 (0.25 mm, 0.06). A one\u2010way ANOVA was significant; Tukey's post hoc test confirmed day\u201030 samples differed from other groups (<jats:italic>P<\/jats:italic> &lt; 0.05), consistent across adjacent zones. Zones far from injury remained similar across groups (<jats:italic>P<\/jats:italic> = 0.143 to 0.551). These measurements matched qualitative histologic variations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Quantifiable VF biomechanical properties can be linked to histology. This technological approach is the first to simultaneously correlate functional biomechanics with histology and is ideal for future preclinical studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 130:454\u2013459, 2020<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27999","type":"journal-article","created":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T22:14:43Z","timestamp":1557180883000},"page":"454-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Quantifying vocal fold wound\u2010healing biomechanical property changes"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4389-3104","authenticated-orcid":false,"given":"Gregory R.","family":"Dion","sequence":"first","affiliation":[{"name":"Dental and Craniofacial Trauma Research Department U.S. Army Institute of Surgical Research  JBSA Fort Sam Houston, San Antonio Texas"},{"name":"Department of Biomedical Engineering University of Texas at San Antonio  San Antonio Texas"},{"name":"NYU Voice Center, Department of Otolaryngology\u2013Head and Neck Surgery New York University School of Medicine  New York New York U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teja","family":"Guda","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Texas at San Antonio  San Antonio Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeyuki","family":"Mukudai","sequence":"additional","affiliation":[{"name":"NYU Voice Center, Department of Otolaryngology\u2013Head and Neck Surgery New York University School of Medicine  New York New York U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Bing","sequence":"additional","affiliation":[{"name":"NYU Voice Center, Department of Otolaryngology\u2013Head and Neck Surgery New York University School of Medicine  New York New York U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Francois","family":"Lavoie","sequence":"additional","affiliation":[{"name":"Biomomentum Inc.  Laval Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1190-9036","authenticated-orcid":false,"given":"Ryan C.","family":"Branski","sequence":"additional","affiliation":[{"name":"NYU Voice Center, Department of Otolaryngology\u2013Head and Neck Surgery New York University School of Medicine  New York New York U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0892-1997(02)00078-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2003.06.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.04.018"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2014.03.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2013.12.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2009.09.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26963"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26410"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27341"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940511400105"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26648"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27047"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200304000-00007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2004.12.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e3181379c7c"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459980112400602"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.27999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.27999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.27999","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.27999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T15:48:55Z","timestamp":1692632935000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.27999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,6]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/lary.27999"],"URL":"https:\/\/doi.org\/10.1002\/lary.27999","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,6]]},"assertion":[{"value":"2018-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}