{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T21:11:05Z","timestamp":1773436265403,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T00:00:00Z","timestamp":1556150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC011818"],"award-info":[{"award-number":["R01 DC011818"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Otitis media with effusion (OME) is a common disease of childhood that is largely asymptomatic. However, middle ear fluid can persist for months and negatively impact a child's quality of life. Many cases of OME remain chronic and require surgical intervention. Because biofilms are known to contribute to the persistence of many diseases, this study examined effusions collected from children with chronic OME for the presence of essential biofilm structural components, members of the DNABII family of bacterial DNA\u2010binding proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Middle ear effusions were recovered from 38 children with chronic OME at the time of tympanostomy tube insertion. A portion of each specimen was submitted for microbiology culture. The remaining material was assessed by immunoblot to quantitate individual DNABII proteins, integration host factor (IHF), and histone\u2010like protein (HU).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty\u2010five percent of effusions (24 of 37) were culture\u2010positive for bacterial species or yeast, whereas 35% (13 of 37) were culture\u2010negative. IHF was detected in 95% (36 of 38) at concentrations from 2 to 481 ng\/\u03bcL effusion. HU was detected in 95% (36 of 38) and quantitated from 13 to 5,264 ng\/\u03bcL effusion (<jats:italic>P<\/jats:italic> \u2264 0.05 compared to IHF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Because DNABII proteins are essential structural components of bacterial biofilms, these data lend further support to our understanding that biofilms are present in the vast majority of chronic middle ear effusions, despite negative culture results. The presence and ubiquity of DNABII proteins in OME specimens indicated that these proteins can serve as an important clinical target for our novel DNABII\u2010directed strategy to treat biofilm diseases such as chronic OME.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA<\/jats:p><jats:p><jats:italic>Laryngoscope<\/jats:italic>, 130:806\u2013811, 2020<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.28011","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T15:48:25Z","timestamp":1556207305000},"page":"806-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7248-8979","authenticated-orcid":false,"given":"Christine L.","family":"Barron","sequence":"first","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"Department of Pediatric Otolaryngology\u2013Head and Neck Surgery Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louie B.","family":"Kamel\u2010Abusalha","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"Department of Pediatric Otolaryngology\u2013Head and Neck Surgery Nationwide Children's Hospital  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0425-7682","authenticated-orcid":false,"given":"Rishabh","family":"Sethia","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"Department of Pediatric Otolaryngology\u2013Head and Neck Surgery Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Goodman","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1890-2876","authenticated-orcid":false,"given":"Charles A.","family":"Elmaraghy","sequence":"additional","affiliation":[{"name":"Department of Pediatric Otolaryngology\u2013Head and Neck Surgery Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren O.","family":"Bakaletz","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599815623467"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2273.2000.00350.x"},{"key":"e_1_2_7_4_1","first-page":"1","article-title":"Diagnosis, natural history, and late effects of otitis media with effusion","volume":"2002","author":"Shekelle P","journal-title":"Evid Rep Technol Assess (Summ)"},{"key":"e_1_2_7_5_1","first-page":"15","article-title":"Update on otitis media: prevention and treatment","volume":"7","author":"Qureishi A","year":"2014","journal-title":"Infect Drug Resist"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100128567"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200310000-00004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599813487302"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1995.03520440052036"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5876(01)00510-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1185-1188.1998"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.279.4.296"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.202"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2431-11-94"},{"key":"e_1_2_7_15_1","unstructured":"National Institute of Health: Research on microbial biofilms: PA Number: PA\u201003\u2010047.2002. 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