{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:28:06Z","timestamp":1789939686813,"version":"4.0.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T00:00:00Z","timestamp":1560211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether ganciclovir (GCV) treatment reduces sensorineural hearing loss in cytomegalovirus (CMV)\u2010infected mice. The effects of GCV on viral load, absolute neutrophil count (ANC), and outer hair cell (OHC) integrity were also investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Infected BALB\/c mice were inoculated with murine CMV on postnatal day 3. Those treated with GCV received an intraperitoneal injection twice a day for 14 days. Auditory thresholds were assessed using distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) testing 4 weeks after inoculation. Temporal bones were used for determination of viral load by quantitative polymerase chain reaction and hair cell quantification by scanning electron microscopy. ANCs were completed by an automated hematology analyzer, with manual review for confirmation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GCV\u2010treated CMV\u2010infected mice had lower ABR (<jats:italic>P<\/jats:italic> &lt;\u20090.0001, Kruskal\u2010Wallis test) and DPOAE (<jats:italic>P<\/jats:italic> &lt;\u20090.0001) thresholds compared to CMV\u2010infected untreated mice, indicating that GCV protected mice from CMV\u2010induced hearing loss. Viral load in infected populations undergoing GCV treatment was significantly decreased (<jats:italic>P<\/jats:italic> =\u20090.03) relative to untreated mice. GCV treatment alone had no effect on ABR and DPOAE compared to untreated, uninfected controls (<jats:italic>P<\/jats:italic> =\u20090.1, <jats:italic>P<\/jats:italic> =\u20090.24, respectively). GCV\u2010treated mice received increased protection from OHC loss when compared to untreated groups, with total OHC losses of approximately 7% and 14%, respectively (<jats:italic>P<\/jats:italic> &lt;\u20090.05). Neutropenia was absent after 7 days of GCV treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ganciclovir effectively ameliorated SNHL and partially protected from OHC loss in a preclinical model of congenital CMV infection, seemingly by reducing viral load.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 130:1064\u20131069, 2020<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.28134","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T16:26:36Z","timestamp":1560270396000},"page":"1064-1069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of ganciclovir treatment in a murine model of cytomegalovirus\u2010induced hearing loss"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0123-4138","authenticated-orcid":false,"given":"Travis J.","family":"Haller","sequence":"first","affiliation":[{"name":"Division of Otolaryngology\u2013Head and Neck Surgery University of Utah School of Medicine  Salt Lake City Utah U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa S.","family":"Price","sequence":"additional","affiliation":[{"name":"School of Medicine University of Utah School of Medicine  Salt Lake City Utah U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spencer R.","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2013Head and Neck Surgery University of Utah School of Medicine  Salt Lake City Utah U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Hillas","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Seipp","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Firpo","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Utah School of Medicine  Salt Lake City Utah U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert H.","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2013Head and Neck Surgery University of Utah School of Medicine  Salt Lake City Utah U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365540802308431"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.535"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2006-3441"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.09.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000212107"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/014556131609500611"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1404599"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2018.02.028"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002079"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006599"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24090"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(03)00192-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.05.019"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-011-0024-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06423-11"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.28134","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.28134","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.28134","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.28134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T02:21:16Z","timestamp":1694053276000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.28134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,11]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/lary.28134"],"URL":"https:\/\/doi.org\/10.1002\/lary.28134","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,11]]},"assertion":[{"value":"2019-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}