{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T12:15:29Z","timestamp":1783599329287,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T00:00:00Z","timestamp":1585267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005338","name":"Educational and Research Foundation for the American Academy of Facial Plastic and Reconstructive Surgery","doi-asserted-by":"publisher","award":["Leslie Bernstein Resident Research Grant"],"award-info":[{"award-number":["Leslie Bernstein Resident Research Grant"]}],"id":[{"id":"10.13039\/100005338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Facial nerve injury is a source of major morbidity. This study investigated the neuroregenerative effects of inducing an anti\u2010inflammatory environment when reconstructing a facial nerve defect with a multichannel bridge containing interleukin\u20104 (IL\u20104)\u2013encoding lentivirus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen adult Sprague\u2010Dawley rats were divided into three groups, all of which sustained a facial nerve gap defect. Group I had reconstruction performed via an IL\u20104 multichannel bridge, group II had a multichannel bridge with saline placed, and group III had no reconstruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Quantitative histomorphometric data were assessed 10\u2009weeks after injury. On post hoc analysis, the IL\u20104 bridge group demonstrated superior regeneration compared to bridge alone on fiber density (mean = 2,380\u2009\u00b1\u2009297 vs. 1,680\u2009\u00b1\u2009441 fibers\/mm<jats:sup>2<\/jats:sup>, <jats:italic>P<\/jats:italic> =\u2009.05) and latency time (mean = 2.9\u2009ms\u2009\u00b1\u20090.6\u2009ms vs. 3.6\u2009ms\u2009\u00b1\u20090.3\u2009ms, <jats:italic>P<\/jats:italic> &lt;\u2009.001). There was significantly greater regeneration in the IL\u20104 bridge group versus unreconstructed defect for total fiber and density measurements (<jats:italic>P<\/jats:italic> \u2264\u2009.05). Comparison of facial motor\u2010evoked distal latencies between the IL\u20104 bridge group versus bridge alone revealed significant electrophysiological improvement at week 8 (<jats:italic>P<\/jats:italic> =\u2009.02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Inflammation has been implicated in a variety of otolaryngologic disorders. This study demonstrates that placement of a multichannel bridge with lentivirus encoding IL\u20104 improves regenerative outcomes following facial nerve gap injury in rodents. This effect is likely mediated by promotion of an anti\u2010inflammatory environment, and these findings may inform future therapeutic approaches to facial nerve injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 2020<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.28629","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T14:22:00Z","timestamp":1585318920000},"page":"2802-2810","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Delivery of Interleukin\u20104\u2013Encoding Lentivirus Using Multiple\u2010Channel Bridges Enhances Nerve Regeneration"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7922-4426","authenticated-orcid":false,"given":"S. Ahmed","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3433-1874","authenticated-orcid":false,"given":"John E.","family":"Hanks","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron W.","family":"Stebbins","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osama","family":"Alkhalili","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha T.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Y.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney M.","family":"Dumont","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Michigan Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lonnie 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