{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T13:56:27Z","timestamp":1770645387590,"version":"3.49.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T00:00:00Z","timestamp":1590019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["NCATS UL1TR002537"],"award-info":[{"award-number":["NCATS UL1TR002537"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective was to explore the effect of the proneuronal transcription factor neurogenic differentiation 1 (Neurod1, ND1) on Schwann cells (SC) and schwannoma cell proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using a variety of transgenic mouse lines, we investigated how expression of Neurod1 effects medulloblastoma (MB) growth, schwannoma tumor progression, vestibular function, and SC cell proliferation. Primary human vestibular schwannoma (VS) cell cultures were transduced with adenoviral vectors expressing Neurod1. Cell proliferation was assessed by 5\u2010ethynyl\u20102'\u2010deoxyuridine (EdU) uptake.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Basic science investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of Neurod1 reduced the growth of slow\u2010growing but not fast\u2010growing MB models. Gene transfer of Neurod1 in human schwannoma cultures significantly reduced cell proliferation in dose\u2010dependent way. Deletion of the neurofibromatosis type 2 (<jats:italic>Nf2)<\/jats:italic> tumor\u2010suppressor gene via Cre expression in SCs led to increased intraganglionic SC proliferation and mildly reduced vestibular sensory\u2010evoked potentials (VsEP) responses compared to age\u2010matched wild\u2010type littermates. The effect of Neurod1\u2010induced expression on intraganglionic SC proliferation in animals lacking <jats:italic>Nf2<\/jats:italic> was mild and highly variable. Sciatic nerve axotomy significantly increased SC proliferation in wild\u2010type and <jats:italic>Nf2<\/jats:italic>\u2010null animals, and expression of Neurod1 reduced the proliferative capacity of both wild\u2010type and <jats:italic>Nf2<\/jats:italic>\u2010null SCs following nerve injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Expression of Neurod1 reduces slow\u2010growing MB progression and reduces human SC proliferation in primary VS cultures. In a genetic mouse model of schwannomas, we find some effects of Neurod1 expression; however, the high variability indicates that more tightly regulated Neurod1 expression levels that mimic our in vitro data are needed to fully validate Neurod1 effects on schwannoma progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 131:E259\u2013E270, 2021<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.28671","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T22:43:14Z","timestamp":1590100994000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effects of Neurod1 Expression on Mouse and Human Schwannoma Cells"],"prefix":"10.1002","volume":"131","author":[{"given":"Jennifer","family":"Kersigo","sequence":"first","affiliation":[{"name":"Department of Biology University of Lowa  Lowa City Lowa U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lintao","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology University of Lowa  Lowa City Lowa U.S.A."},{"name":"Decibel Pharmaceutical  Boston Massachusetts U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linjing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology University of Lowa  Lowa City Lowa U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Biology University of Lowa  Lowa City Lowa U.S.A."},{"name":"Department of Special Education &amp; Communication Disorders University of Nebraska  Lincoln Nebraska U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarath","family":"Vijayakuma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated 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