{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:07:05Z","timestamp":1789168025914,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T00:00:00Z","timestamp":1596844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81370113"],"award-info":[{"award-number":["81370113"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP and NECRSwNP) show distinguished clinical pathology, but their underlying mechanism remains unclear. We aimed to investigate the clinical, hematological, and histopathological changes in chronic rhinosinusitis with nasal polyps (CRSwNP) endotypes and its association with microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>A comparative cross\u2010sectional study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A comparative study of 46 patients with CRSwNP (34.69\u2009\u00b1\u200916.39\u2009years old) who underwent endoscopic sinus surgery were recruited and subdivided into ECRSwNP and NECRSwNP groups based on eosinophilic tissue inflammation; 12 healthy controls were also included. A structured histopathological analysis was conducted, and complete blood count was determined in patients. Endoscopic\u2010guided middle meatus swabs and fecal samples were collected from the patients and controls and subsequently subjected to 16S rRNA gene sequencing on Illumina MiSeq.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to NECRSwNP, ECRSwNP showed a statistically significant increase in the computed tomography score, endoscopic score, blood eosinophil percentage, tissue eosinophil count, inflammation degree, subepithelial edema, and eosinophil aggregation. Airway microbiota communities differed among the three groups. The abundance of <jats:italic>Moraxella<\/jats:italic> and <jats:italic>Parvimonas<\/jats:italic> was significantly higher in the ECRSwNP group. Distinct microbiota dysbiosis in CRSwNP endotypes was found to be correlated with different clinical pathologies. Moreover, the gut microbiota in ECRSwNP and NECRSwNP showed dysbiosis, that is, significant decrease in the abundance of <jats:italic>Actinobacteria<\/jats:italic> in the former and significant increase in the abundance of <jats:italic>Enterobacterales<\/jats:italic> and several genera in NECRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Significant clinical pathology and microbiota changes were evident in patients with ECRSwNP and NECRSwNP. Distinct microbiota dysbiosis was correlated with different clinical pathologies. Understanding these differences may improve the prognosis and treatment of chronic rhinosinusitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>4 <jats:italic>Laryngoscope<\/jats:italic>, 131:E34\u2013E44, 2021<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.28858","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T14:54:17Z","timestamp":1596898457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Distinct Clinical Pathology and Microbiota in Chronic Rhinosinusitis With Nasal Polyps Endotypes"],"prefix":"10.1002","volume":"131","author":[{"given":"Elrayah E.","family":"Abbas","sequence":"first","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"},{"name":"Department of Microbiology Military Hospital  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman","family":"Elfadil","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Military Hospital  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3343-9605","authenticated-orcid":false,"given":"Shu","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Microecology Collage of Basic Medical Science, Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2016.12.018"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02477"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-052016-100401"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2018.10.014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2016.06.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22214"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-015-0579-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26327-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2015.01.019"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2016.04.012"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2017.05.019"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2018.09.005"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-019-0350-y"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21304"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2018.03.020"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/1945892418779451"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-018-0800-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000147917.43615.c0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v2.i12.873"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22507"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22314"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016489.2018.1538567"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21943"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0000000000000510"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22192"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00060-16"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.05.050"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.142"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21871"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2018.05.009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0000000000000221"},{"key":"e_1_2_7_33_1","article-title":"European position paper on rhinosinusitis and nasal polyps 2012","volume":"23","author":"Fokkens WJ","year":"2012","journal-title":"Rhinol Suppl"},{"key":"e_1_2_7_34_1","first-page":"183","article-title":"Staging in rhinosinusitus","volume":"31","author":"Lund VJ","year":"1993","journal-title":"Rhinology"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-59989770005-6"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2009.10.005"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2017.06.008"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-014-3174-4"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27726"},{"key":"e_1_2_7_40_1","article-title":"Microbiotyping the sinonasal microbiome","author":"Bassiouni A","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2015.05.013"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.02.007"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.06.002"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2015.10.007"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2017.06.012"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6657(11)70680-1"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000016911"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.14"},{"key":"e_1_2_7_49_1","volume-title":"Bailey & Scott's Diagnostic Microbiology E\u2010Book","author":"Tille P.","year":"2014"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.496"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00187-18"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.03.044"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.10.046"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00150-17"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2016.07.001"},{"key":"e_1_2_7_56_1","first-page":"2492","article-title":"Establishment of different experimental asthma models in mice","volume":"15","author":"Yu QL","year":"2018","journal-title":"Exp Ther Med"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13052"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201503-133AW"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147809"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.111237"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.02.004"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.28858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.28858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.28858","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.28858","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T15:11:52Z","timestamp":1693581112000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.28858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,8]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/lary.28858"],"URL":"https:\/\/doi.org\/10.1002\/lary.28858","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,8]]},"assertion":[{"value":"2019-12-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}