{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T02:00:06Z","timestamp":1783994406123,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T00:00:00Z","timestamp":1606521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC014070\u201001A1"],"award-info":[{"award-number":["R01DC014070\u201001A1"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec><jats:title>Objective\/Hypothesis<\/jats:title><jats:p>There are currently no treatments available that restore dynamic laryngeal function after hemilaryngectomy. We have shown that dynamic function can be restored post hemilaryngectomy in a rat model. Here, we report in a first of its kind, proof of concept study that this previously published technique is scalable to a porcine model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Muscle and fat biopsies were taken from three Yucatan minipigs. Muscle progenitor cells (MPCs) and adipose stem cells (ASCs) were isolated and cultured for 3\u2009weeks. The minipigs underwent a left laterovertical partial laryngectomy sparing the left arytenoid cartilage and transecting the recurrent laryngeal nerve. Each layer was replaced with a tissue\u2010engineered implant: 1) an acellular mucosal layer composed of densified Type I oligomeric collagen, 2) a skeletal muscle layer composed of autologous MPCs and aligned oligomeric collagen differentiated and induced to express motor endplates (MEE), and 3) a cartilage layer composed of autologous ASCs and densified oligomeric collagen differentiated to cartilage. Healing was monitored at 2 and 4\u2009weeks post\u2010op, and at the 8\u2009week study endpoint.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Animals demonstrated appropriate weight gain, no aspiration events, and audible phonation. Video laryngoscopy showed progressive healing with vascularization and re\u2010epithelialization present at 4\u2009weeks. On histology, there was no immune reaction to the implants and there was complete integration into host tissue with nerve and vascular ingrowth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This pilot study represents a first in which a transmural vertical partial laryngectomy was performed and successfully repaired with a customized, autologous stem cell\u2010derived multi\u2010layered tissue\u2010engineered implant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 131:2277\u20132284, 2021<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.29282","type":"journal-article","created":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T14:10:07Z","timestamp":1606572607000},"page":"2277-2284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Laryngeal Reconstruction Using Tissue\u2010Engineered Implants in Pigs: A Pilot Study"],"prefix":"10.1002","volume":"131","author":[{"given":"Sarah","family":"Brookes","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Speech, Language and Hearing Sciences Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore J.","family":"Puls","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Kincaid","sequence":"additional","affiliation":[{"name":"Department of Neurology Indiana University School of Medicine (IUSM)  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry","family":"Voytik\u2010Harbin","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences Purdue University  West Lafayette Indiana U.S.A."},{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-4403","authenticated-orcid":false,"given":"Stacey","family":"Halum","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,28]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"SEER Stat Fact Sheets:Larynx Cancer USA: National Cancer Institute2018. Available at:https:\/\/seer.cancer.gov\/statfacts\/html\/laryn.html."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1992.01880060046013"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31803e8c8d"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31816c438e"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23519"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1598"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489414523709"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26771"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26992"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27575"},{"key":"e_1_2_6_12_1","unstructured":"U.S. utility patent application filed for PRF Ref. No. 67585 \"Primed muscle progenitor cells and uses\" (67585\u201302) with the United States Patent & Trademark Office (USPTO) on April 27 2018"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5BM00443H"},{"key":"e_1_2_6_14_1","unstructured":"Collagen Preparation and Method of Isolation. Inventors:Voytik\u2010Harbin S. Kreger S. Bell B. andBailey J. Collagen Preparation and Method of Isolation. U.S. Patent 8 084 055 issued December 27 2011 U.S. Patent 8 512 756 issued August 20 2013."},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21537"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21431"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201503971"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26980"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/08941939509031607"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199912150-00006"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.23621"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2016-0130"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24924"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.29282","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.29282","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.29282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T08:02:10Z","timestamp":1692950530000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.29282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,28]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1002\/lary.29282"],"URL":"https:\/\/doi.org\/10.1002\/lary.29282","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,28]]},"assertion":[{"value":"2020-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}