{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:47:02Z","timestamp":1760986022610,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Test a new jellyfish collagen biomaterial aimed to increase duration of injection medialization laryngoplasty (IL) against two products in clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N\u00a0=\u00a06\/group). Group 1 received micronized cross\u2010linked jellyfish collagen (MX\u2010JC) and adipose derived stem cells (ADSCs), Group 2, MX\u2010JC alone, Group 3, cross\u2010linked hyaluronic acid (X\u2010HA), and Group 4, micronized acellular dermis (MACD). Animals were sacrificed at 4 and 12\u2009weeks. Major outcomes were MRI tissue volumes and histopathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 100\u2009\u03bcL IL MRI volumes (means \u00b1 STD) at 4 and 12\u2009weeks were: Group 1: 27.2\u2009\u00b1\u200915.6 and 13.1\u2009\u00b1\u20095.2\u2009\u03bcL, Group 2: 60.8\u2009\u00b1\u200918 and 27.8\u2009\u00b1\u20092.47\u2009\u03bcL, Group 3: 27.4\u2009\u00b1\u200912 and 10.6\u2009\u00b1\u20098\u2009\u03bcL, and Group 4: 37.5\u2009\u00b1\u200911 and 9.85\u2009\u00b1\u20091\u2009\u03bcL. Group 2 volumes were largest and Group 3 were smallest in all comparisons (<jats:italic>P<\/jats:italic>\u00a0&lt;\u2009.05). Histologically, low grade inflammatory responses were observed in Group 1, mild histiocytic infiltration in Group 2, widespread muscle fiber loss in Group 3, and plasmocytic infiltration in Group 4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MX\u2010JC showed the least resorption at 4 and 12\u2009weeks among all groups. T cell inflammatory responses were observed with MX\u2010JC but were reduced by 12\u2009weeks while B cell immune responses, indicative of antibody priming, were predominantly noted with MACD. MX\u2010JC\u2009+\u2009ADSC showed low grade immunity while the XHA showed greater myocyte loss compared to the other groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 131:E2452\u2013E2460, 2021<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.29501","type":"journal-article","created":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:14:33Z","timestamp":1618359273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>MRI<\/scp> Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty"],"prefix":"10.1002","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5312-4774","authenticated-orcid":false,"given":"Serban","family":"San\u2010Marina","sequence":"first","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Bowen","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5419-7748","authenticated-orcid":false,"given":"Michael S.","family":"Oldenburg","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8010-961X","authenticated-orcid":false,"given":"Stephen G.","family":"Voss","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle E.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slobodan","family":"Macura","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology &amp; Metabolomics Core Mayo Clinic  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Meloche","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology &amp; Metabolomics Core Mayo Clinic  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"Alen Lee","family":"Miller","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Sarcoma Biology Laboratory Mayo Clinic  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew Mearns","family":"Spragg","sequence":"additional","affiliation":[{"name":"Jellagen Pty Ltd.  Cardiff United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8770-8463","authenticated-orcid":false,"given":"Dale C.","family":"Ekbom","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599814531733"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.08.054"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3342\/ceo.2010.3.4.177"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000232436.91409.30"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27618"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18523-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8917913"},{"key":"e_1_2_9_9_1","article-title":"Preliminary results of tissue\u2010engineered injection laryngoplasty material in a rabbit model","volume":"128","author":"Oldenburg MS","year":"2017","journal-title":"Laryngoscope"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26262"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33959"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02344779"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169731"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27429"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623319873974"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.4780110811"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489418805503"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31802e9291"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200409000-00018"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480410017701"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200207000-00017"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.01.015"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/md9060967"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00979"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-014-1652-8"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.29501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.29501","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.29501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T22:26:37Z","timestamp":1693347997000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.29501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,13]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/lary.29501"],"URL":"https:\/\/doi.org\/10.1002\/lary.29501","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"type":"print","value":"0023-852X"},{"type":"electronic","value":"1531-4995"}],"subject":[],"published":{"date-parts":[[2021,4,13]]},"assertion":[{"value":"2020-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}