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The complete loss of function in the PTEN\u2010induced kinase 1 gene (<jats:italic>Pink1<\/jats:italic>) causes a genetic form of early\u2010onset, autosomal recessive PD. Modeled after the human inherited mutation, the <jats:italic>Pink1<\/jats:italic>\u2212\/\u2212 rat demonstrates significant cranial sensorimotor dysfunction including declines in ultrasonic vocalizations. However, the underlying genetics of the vocal fold thyroarytenoid (TA) muscle that may contribute to vocal deficits has not been studied. The aim of this study was to identify differentially expressed genes in the TA muscle of 8\u2010month\u2010old male <jats:italic>Pink1\u2212\/\u2212<\/jats:italic> rats compared to wildtype controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal experiment with control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>High throughput RNA sequencing was used to examine TA muscle gene expression in adult male <jats:italic>Pink1<\/jats:italic>\u2212\/\u2212 rats and wildtype controls. Weighted Gene Co\u2010expression Network Analysis was used to construct co\u2010expression modules to identify biological networks, including where <jats:italic>Pink1<\/jats:italic> was a central node. The ENRICHR tool was used to compare this gene set to existing human gene databases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 134 annotated differentially expressed genes (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05 cutoff) and observed enrichment in the following biological pathways: Parkinson's disease (<jats:italic>Casp7<\/jats:italic>, <jats:italic>Pink1<\/jats:italic>); Parkin\u2010Ubiquitin proteasome degradation (<jats:italic>Psmd12<\/jats:italic>, <jats:italic>Psmd7<\/jats:italic>); MAPK signaling (<jats:italic>Casp7<\/jats:italic>, <jats:italic>Ppm1b<\/jats:italic>, <jats:italic>Ppp3r1<\/jats:italic>); and inflammatory TNF\u2010\u03b1, Nf\u2010\u03baB Signaling (<jats:italic>Casp7<\/jats:italic>, <jats:italic>Psmd12<\/jats:italic>, <jats:italic>Psmd7<\/jats:italic>, <jats:italic>Cdc34<\/jats:italic>, <jats:italic>Bcl7a<\/jats:italic>, <jats:italic>Peg3<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Genes and pathways identified here may be useful for evaluating the specific mechanisms of peripheral dysfunction including within the laryngeal muscle and have potential to be used as experimental biomarkers for treatment development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 131:E2874\u2013E2879, 2021<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.29661","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T12:09:05Z","timestamp":1622462945000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Thyroarytenoid Muscle Gene Expression in a Rat Model of Early\u2010Onset Parkinson's Disease"],"prefix":"10.1002","volume":"131","author":[{"given":"Sarah 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