{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:15:53Z","timestamp":1789820153829,"version":"4.0.1"},"reference-count":74,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Gastroesophageal reflux disease (GERD) and laryngopharyngeal reflux (LPR) are chronic conditions caused by backflow of gastric and duodenal contents into the esophagus and proximal aerodigestive tract, respectively. Mucosal barrier dysfunction resultant from the synergistic actions of chemical injury and the mucosal inflammatory response during reflux contributes to symptom perception. Alginates effectively treat symptoms of mild to moderate GERD and have recently shown benefit for LPR. In addition to forming a \u201craft\u201d over gastric contents to reduce acidic reflux episodes, alginates have been found to bind the esophageal mucosa thereby preserving functional barrier integrity measured by transepithelial electrical resistance. The aim of this study was to further examine the topical protective capacity of alginate\u2010based Gaviscon Advance (GA) and Double Action (GDA) against pepsin\u2010acid mediated aerodigestive epithelial barrier dysfunction in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Translational.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immortalized human esophageal and vocal cord epithelial cells cultured in transwells were pretreated with liquid formula GA, GDA, matched viscous placebo solution, or saline (control), then treated for 1\u2009h with saline, acid (pH\u00a03\u20136) or pepsin (0.1\u20131\u00a0mg\/ml) at pH\u00a03\u20136. Endpoint measure was taken of horseradish peroxidase (HRP) allowed to diffuse across monolayers for 2\u2009h.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Pepsin (0.1\u20101\u00a0mg\/ml) at pH\u00a03\u20106 increased HRP flux through cultures pretreated with saline or placebo (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u20090.05); acid alone did not. GA and GDA prevented barrier dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      GA and GDA preserved epithelial barrier function during pepsin\u2010acid insult better than placebo suggesting that protection was due to alginate. These data support topical protection as a therapeutic approach to GERD and LPR.\n                      <jats:italic>Laryngoscope<\/jats:italic>\n                      , 132:2327\u20132334, 2022\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.30087","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T08:27:05Z","timestamp":1646296025000},"page":"2327-2334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Alginates for Protection Against\n                    <scp>Pepsin\u2010Acid<\/scp>\n                    Induced Aerodigestive Epithelial Barrier Disruption"],"prefix":"10.1002","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7045-357X","authenticated-orcid":false,"given":"Tina L.","family":"Samuels","sequence":"first","affiliation":[{"name":"Otolaryngology and Communication Sciences Medical College of Wisconsin  Milwaukee Wisconsin U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yan","sequence":"additional","affiliation":[{"name":"Pediatrics Quantitative Health Sciences Medical College of Wisconsin  Milwaukee Wisconsin U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishma","family":"Patel","sequence":"additional","affiliation":[{"name":"Reckitt Benckiser  Hull, England United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Plehhova","sequence":"additional","affiliation":[{"name":"Reckitt Benckiser  Hull, England United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathal","family":"Coyle","sequence":"additional","affiliation":[{"name":"Reckitt Benckiser  Hull, England United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan P.","family":"Hurley","sequence":"additional","affiliation":[{"name":"Pediatrics, Mucosal Immunology &amp; Biology Research 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