{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T05:03:39Z","timestamp":1783832619718,"version":"3.55.0"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007760","name":"College of Medicine Office of Research, Ohio State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007760","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NHLBI K08HL138460"],"award-info":[{"award-number":["NHLBI K08HL138460"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NHLBI R01HL157039"],"award-info":[{"award-number":["NHLBI R01HL157039"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Partially decellularized tracheal scaffolds have emerged as a potential solution for long\u2010segment tracheal defects. These grafts have exhibited regenerative capacity and the preservation of native mechanical properties resulting from the elimination of all highly immunogenic cell types while sparing weakly immunogenic cartilage. With partial decellularization, new considerations must be made about the viability of preserved chondrocytes. In this study, we propose a multimodal approach for quantifying chondrocyte viability for airway tissue engineering.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tracheal segments (5\u2009mm) were harvested from<jats:styled-content style=\"fixed-case\">C57BL<\/jats:styled-content>\/6 mice, and immediately stored in phosphate\u2010buffered saline at \u221220\u00b0C (<jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>\u201020) or biobanked via cryopreservation. Stored and control (fresh) tracheal grafts were implanted as syngeneic tracheal grafts (<jats:styled-content style=\"fixed-case\">STG<\/jats:styled-content>) for 3\u2009months.<jats:styled-content style=\"fixed-case\">STG<\/jats:styled-content>was scanned with micro\u2010computed tomography (<jats:styled-content style=\"fixed-case\">\u03bcCT<\/jats:styled-content>) in vivo.<jats:styled-content style=\"fixed-case\">STG<\/jats:styled-content>subjected to different conditions (fresh,<jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>\u201020, or biobanked) were characterized with live\/dead assay, terminal deoxynucleotidyl transferase<jats:styled-content style=\"fixed-case\">dUTP<\/jats:styled-content>nick end labeling (<jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content>), and von Kossa staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Live\/dead assay detected higher chondrocyte viability in biobanked conditions compared to PBS\u201020. TUNEL staining indicated that storage conditions did not alter the proportion of apoptotic cells. Biobanking exhibited a lower calcification area than PBS\u201020 in 3\u2010month post\u2010implanted grafts. Higher radiographic density (Hounsfield units) measured by \u03bcCT correlated with more calcification within the tracheal cartilage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We propose a strategy to assess chondrocyte viability that integrates with vivo imaging and histologic techniques, leveraging their respective strengths and weaknesses. These techniques will support the rational design of partially decellularized tracheal scaffolds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A<jats:italic>Laryngoscope<\/jats:italic>, 133:512\u2013520, 2023<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.30206","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T13:34:11Z","timestamp":1653485651000},"page":"512-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Multimodal Approach to Quantify Chondrocyte Viability for Airway Tissue Engineering"],"prefix":"10.1002","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9240-3883","authenticated-orcid":false,"given":"Coreena","family":"Chan","sequence":"first","affiliation":[{"name":"College of Medicine The Ohio State University Columbus Ohio 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