{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:39:50Z","timestamp":1779147590615,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,7,8]],"date-time":"2023-07-08T00:00:00Z","timestamp":1688774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Compare hearing outcomes utilizing standard, prolonged and delayed ganciclovir (GCV) therapy in a murine model of cytomegalovirus (CMV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BALB\/c mice were inoculated with mouse cytomegalovirus (mCMV) or saline via intracerebral injection on postnatal day 3 (p3). Intraperitoneal GCV or saline was administered at 12\u2009h intervals for the duration of the standard (p3\u2010p17), delayed (p30\u2010p44), or prolonged treatment windows (p3\u2010p31). Auditory thresholds were assessed using distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) testing at 4, 6, and 8\u2009weeks of age. Blood and tissue samples were harvested from mice on p17 and p37 one hour after GCV administration, and their concentrations were assessed via liquid chromatography\u2010mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A delayed course of GCV improved ABR but not DPOAE thresholds in mCMV\u2010infected mice. A prolonged course of GCV did not provide better hearing thresholds than those administered standard treatment. The average GCV concentration in all 17\u2010day\u2010old mice tissue was significantly higher than those in older 37\u2010day\u2010old mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Delayed GCV treatment provided a hearing benefit on ABR over untreated mCMV infected mice. Prolonged CGV administration showed no benefit compared to a shorter duration GCV treatment. GCV drug concentrations both systemically and in the cochlea are much lower in older mice. These results have potential implications for the clinical management of cCMV infected children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 134:433\u2013438, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.30860","type":"journal-article","created":{"date-parts":[[2023,7,8]],"date-time":"2023-07-08T10:46:13Z","timestamp":1688813173000},"page":"433-438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hearing Following Prolonged and Delayed Ganciclovir Treatment in a Murine Cytomegalovirus Model"],"prefix":"10.1002","volume":"134","author":[{"given":"Katrina","family":"Hillam","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Suarez","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology State University of New York Downstate Medical Center  Stony Brook New York U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Nielson","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail","family":"Traxler","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Sommer","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Winslow","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Holley","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0042-4976","authenticated-orcid":false,"given":"Emily","family":"Huang","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maura","family":"Hughes","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew A.","family":"Firpo","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Rower","sequence":"additional","affiliation":[{"name":"University of Utah Center for Human Toxicology and Department of Pharmacology and Toxicology  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9089-3014","authenticated-orcid":false,"given":"Albert H.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Utah  Salt Lake City Utah U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.535"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(03)00192-6"},{"key":"e_1_2_6_4_1","unstructured":"KimberlinDW JesterP SanchezPJ AhmedA Arav\u2010BogerR.Six Months Versus Six Weeks of Oral Valganciclovir for Infants with Symptomatic Congenital Cytomegalovirus (CMV) Disease with and without Central Nervous System (CNS) Involvement: Results of a Phase III Randomized Double\u2010Blind Placebo\u2010Controlled Multinational StudyID Week. 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