{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:46:31Z","timestamp":1790786791692,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:00:00Z","timestamp":1691107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the utility of 5\u2010aminolevulinic acid (5\u2010ALA) fluorescence for resection of head and neck carcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this prospective pilot trial, 5\u2010ALA was administered as an oral suspension 3\u20135\u2009h prior to induction of anesthesia for resection of head and neck squamous cell carcinoma (HNSCC). Following resection, 405\u2009nm blue light was applied, and fluorescence of the tumor as well as the surgical bed was recorded. Specimen fluorescence intensity was graded categorically as none (score\u2009=\u20090), mild (1), moderate (2), or robust (3) by the operating surgeon intraoperatively and corroborated with final pathologic diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven patients underwent resection with 5\u2010ALA. Five (83%) were male with an age range of 33\u201382\u2009years (mean\u2009=\u200960). Sites included nasal cavity (<jats:italic>n<\/jats:italic>\u2009=\u20093), oral cavity (<jats:italic>n<\/jats:italic>\u2009=\u20093), and the larynx (<jats:italic>n<\/jats:italic>\u2009=\u20091). All specimens demonstrated robust fluorescence when 5\u2010ALA was administered 3\u20135\u2009h preoperatively. 5\u2010ALA fluorescence predicted the presence of perineural invasion, a positive margin, and metastatic lymphadenopathy. Two patients had acute photosensitivity reactions, and one patient had a temporary elevation of hepatic enzymes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>5\u2010ALA induces robust intraoperative fluorescence of HNSCC, capable of demonstrating a positive margin, perineural invasion, and metastatic nodal disease. Although no conclusions are there about the safety of this drug in the head and neck cancer population, our study parallels the extensive safety data in the neurosurgical literature. Future applications may include intraoperative assessment of margin status, diagnostic accuracy, and impacts on survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>4 <jats:italic>Laryngoscope<\/jats:italic>, 134:741\u2013748, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.30910","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T13:11:48Z","timestamp":1691154708000},"page":"741-748","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<scp>5\u2010Aminolevulinic<\/scp> Acid <scp>Fluorescence\u2010Guided<\/scp> Surgery in Head and Neck Squamous Cell Carcinoma"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6898-6633","authenticated-orcid":false,"given":"Peter","family":"Filip","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0431-2920","authenticated-orcid":false,"given":"David K.","family":"Lerner","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8041-960X","authenticated-orcid":false,"given":"Evan","family":"Kominsky","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Schupper","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazir Mohemmed","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Roof","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantinos","family":"Hadjipanayis","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Genden","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Icahn School of Medicine at Mount Sinai  New York City New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred M. 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