{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:57:38Z","timestamp":1790377058841,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T00:00:00Z","timestamp":1691971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NHLBI K08HL138460"],"award-info":[{"award-number":["NIH NHLBI K08HL138460"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NHLBI R01HL157039"],"award-info":[{"award-number":["NIH NHLBI R01HL157039"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Composite tracheal grafts (CTG) combining decellularized scaffolds with external biomaterial support have been shown to support host\u2010derived neotissue formation. In this study, we examine the biocompatibility, graft epithelialization, vascularization, and patency of three prototype CTG using a mouse microsurgical model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Tracheal replacement, regenerative medicine, biocompatible airway splints, animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>CTG electrospun splints made by combining partially decellularized tracheal grafts (PDTG) with polyglycolic acid (PGA), poly(lactide\u2010co\u2010\u03b5\u2010caprolactone) (PLCL), or PLCL\/PGA were orthotopically implanted in mice (<jats:italic>N<\/jats:italic>\u2009=\u200910\/group). Tracheas were explanted two weeks post\u2010implantation. Micro\u2010Computed Tomography was conducted to assess for graft patency, and histological analysis was used to assess for epithelialization and neovascularization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Most animals (greater than 80%) survived until the planned endpoint and did not exhibit respiratory symptoms. MicroCT confirmed the preservation of graft patency. Grossly, the PDTG component of CTG remained intact. Examining the electrospun component of CTG, PGA degraded significantly, while PLCL+PDTG and PLCL\/PGA\u2009+\u2009PDTG maintained their structure. Microvasculature was observed across the surface of CTG and infiltrating the pores. There were no signs of excessive cellular infiltration or encapsulation. Graft microvasculature and epithelium appear similar in all groups, suggesting that CTG did not hinder endothelialization and epithelialization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found that all electrospun nanofiber CTGs are biocompatible and did not affect graft patency, endothelialization and epithelialization. Future directions will explore methods to accelerate graft regeneration of CTG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A <jats:italic>Laryngoscope<\/jats:italic>, 134:1155\u20131162, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.30955","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T12:50:24Z","timestamp":1692017424000},"page":"1155-1162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Assessing the Biocompatibility and Regeneration of Electrospun\u2010Nanofiber Composite Tracheal Grafts"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3921-4447","authenticated-orcid":false,"given":"Lily","family":"Kreber","sequence":"first","affiliation":[{"name":"College of Medicine The Ohio State University  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9849-6881","authenticated-orcid":false,"given":"Lumei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayali","family":"Dharmadhikari","sequence":"additional","affiliation":[{"name":"College of Medicine The Ohio State University  Columbus Ohio U.S.A."},{"name":"Center for Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-464X","authenticated-orcid":false,"given":"Zheng Hong","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Medicine The Ohio State University  Columbus Ohio U.S.A."},{"name":"Department of Otolaryngology \u2010 Head and Neck Surgery Nationwide Children's Hospital  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coreena","family":"Chan","sequence":"additional","affiliation":[{"name":"College of Medicine The Ohio State University  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joey","family":"Huddle","sequence":"additional","affiliation":[{"name":"Nanofiber Solutions, Inc.  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