{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T12:59:23Z","timestamp":1774270763571,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006630","name":"Utah State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006630","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Determine whether combination therapy with ganciclovir (GCV) and a Quercetin\u2010P188 solution improves hearing outcomes in a murine cytomegalovirus (CMV) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BALB\/c mice were infected with murine CMV on postnatal day 3 (p3). Quercetin was solubilized in saline using P188 (QP188). Treatment groups received either GCV, QP188, GCV and QP188, or P188 delivery vehicle BID at 12\u2010hour intervals via intraperitoneal injection. All treatment groups were treated for 14\u2009days starting at p3. Uninfected controls were treated with the combined regimen, saline or P188 delivery vehicle. Auditory thresholds were assessed using distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) testing at 4, 6, and 8\u2009weeks of age. Temporal bones from separate CMV\u2010infected groups were harvested at p10, and viral load was determined by quantitative polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CMV\u2010infected mice receiving combination therapy GCV+QP188 demonstrated statistically significant lower ABR (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and DPOAE thresholds (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) compared with mice treated with GCV monotherapy, QP188 monotherapy, and P188 delivery vehicle at 4, 6, and 8\u2009weeks of age. GCV+QP188 combination therapy, GCV monotherapy, and QP188 monotherapy resulted in a nonsignificant reduction in mean viral titers compared to P188 monotherapy (<jats:italic>p<\/jats:italic>\u2009=\u20090.08).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Combining GCV with the excipients quercetin and P188 effectively ameliorated CMV\u2010induced sensorineural hearing loss in a murine model. This result may be partially explained by a reduction in viral titers in mouse temporal bones that correlate with in vitro studies demonstrating additive antiviral effect in cell culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 134:1457\u20131463, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.30975","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T15:32:15Z","timestamp":1692631935000},"page":"1457-1463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Can Ganciclovir and <scp>Quercetin\u2010P188<\/scp> Ameliorate Cytomegalovirus Induced Hearing Loss?"],"prefix":"10.1002","volume":"134","author":[{"given":"Daniel","family":"Suarez","sequence":"first","affiliation":[{"name":"Division of Otolaryngology \u2013 Head and Neck Surgery, Primary Children's Medical Center University of Utah  Salt 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