{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T21:16:28Z","timestamp":1781298988079,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T00:00:00Z","timestamp":1693612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010834","name":"American Laryngological Association","doi-asserted-by":"publisher","award":["2021"],"award-info":[{"award-number":["2021"]}],"id":[{"id":"10.13039\/100010834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006458","name":"Children's Hospital of Philadelphia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006458","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE 1845298"],"award-info":[{"award-number":["DGE 1845298"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Severe subglottic stenosis develops as a response to intubation in 1% of the &gt;200,000 neonatal intensive care unit infants per year and may require laryngotracheal reconstruction (LTR) with autologous hyaline cartilage. Although effective, LTR is limited by comorbidities, severity of stenosis, and graft integration. In children, there is a significant incidence of restenosis requiring revision surgery. Tissue engineering has been proposed to develop alterative grafting options to improve outcomes and eliminate donor\u2010site morbidity. Our objective is to engineer a decellularized, channel\u2010laden xenogeneic cartilage graft, that we deployed in a proof\u2010of\u2010concept, neonatal porcine LTR model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Meniscal porcine cartilage was freeze\u2013thawed and washed with pepsin\/elastase to decellularize and create microchannels. A 6 \u00d7\u200910\u2010mm decellularized cartilage graft was then implanted in 4 infant pigs in an anterior cricoid split. Airway patency and host response were monitored endoscopically until sacrifice at 12\u2009weeks, when the construct phenotype, cricoid expansion, mechanics, and histomorphometry were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The selective digestion of meniscal components yielded decellularized cartilage with cell\u2010size channels. After LTR with decellularized meniscus, neonatal pigs were monitored via periodic endoscopy observing re\u2010epithelization, integration, and neocartilage formation. At 12\u2009weeks, the graft appeared integrated and exhibited airway expansion of 4\u2009mm in micro\u2010CT and endoscopy. Micro\u2010CT revealed a larger lumen compared with age\u2010matched controls. Finally, histology showed significant neocartilage formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our neonatal porcine LTR model with a decellularized cartilage graft is a novel approach to tissue engineered pediatric LTR. This pilot study sets the stage for \u201coff\u2010the\u2010shelf\u201d graft procurement and future optimization of MEND for LTR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 134:807\u2013814, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31017","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T09:09:55Z","timestamp":1693645795000},"page":"807-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Pilot Study of Decellularized Cartilage for Laryngotracheal Reconstruction in a Neonatal Pig Model"],"prefix":"10.1002","volume":"134","author":[{"given":"Paul M.","family":"Gehret","sequence":"first","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology Children's Hospital of Philadelphia  Philadelphia Pennsylvania U.S.A."},{"name":"Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania  Philadelphia Pennsylvania U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra A.","family":"Dumas","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology Children's Hospital of Philadelphia  Philadelphia Pennsylvania U.S.A."},{"name":"Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania  Philadelphia Pennsylvania U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian N.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology Children's Hospital of Philadelphia  Philadelphia Pennsylvania U.S.A."},{"name":"Department of Otorhinolaryngology Perelman School of Medicine at the University of Pennsylvania  Philadelphia Pennsylvania 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