{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:11:04Z","timestamp":1785305464444,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T00:00:00Z","timestamp":1693612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7172051"],"award-info":[{"award-number":["7172051"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201267"],"award-info":[{"award-number":["82201267"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Muscle RING\u2010finger protein\u20101 (MuRF\u20101), an E3 ubiquitin ligase, has been reported to aggravate skeletal muscle denervated atrophy by mediating the ubiquitination degradation of multiple proteins, whereas the molecular mechanism underlying MuRF\u20101\u2010mediated internal laryngeal muscle denervated atrophy remains unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A rat unilateral recurrent laryngeal nerve (RLN) transection model was established to evaluate denervated muscle atrophy of the larynx. The expression of MuRF\u20101, G\u2010 and F\u2010actin in thyroarytenoid muscle (TA) myocytes before and after RLN injury was analyzed by immunofluorescence and Western blotting. Coimmunoprecipitation experiments detected molecular interactions between MuRF\u20101 and G\u2010actin. Immunoprecipitation tested MuRF\u20101\u2010mediated ubiquitination of G\u2010actin in denervated and innervated TA muscle tissues. The shRNA\u2010MuRF\u20101 AAV was used to suppress MuRF\u20101 expression in denervated TA muscles in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>First, MuRF\u20101 expression was significantly elevated in denervated TA muscle compared to innervated TA muscle (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Second, there was a progressive increase in the G\/F\u2010actin ratio in TA myocytes from day 3 to 14 after RLNI (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Furthermore, colocalization of MuRF\u20101 and G\u2010actin in denervated TA myocytes was observed. Moreover, the upregulation of MuRF\u20101 was closely associated with the ubiquitination of G\u2010actin in denervated TA myocytes and muscle tissues. Knockdown of MuRF\u20101 decelerated the degree of TA muscle atrophy compared with that in the Blank and NC groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) but seemed to promote the compensatory movement of the healthy side.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, we illustrate a novel molecular mechanism underlying MuRF\u20101\u2010mediated internal laryngeal muscle denervated atrophy in that MuRF\u20101 could promote disequilibrium of the G\/F\u2010actin ratio by regulating G\u2010actin ubiquitination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 134:855\u2013864, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31021","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T09:35:50Z","timestamp":1693647350000},"page":"855-864","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>MuRF<\/scp>\u20101 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G\u2010Actin Ubiquitination"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9935-6247","authenticated-orcid":false,"given":"Hong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Beijing Tongren Hospital Capital Medical University  Beijing China"},{"name":"Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education Capital Medical University  Beijing 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