{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:49:21Z","timestamp":1788526161140,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:00:00Z","timestamp":1694476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The effects of electronic cigarettes (e\u2010cigarettes) on the larynx are relatively unknown. This study examined the short\u2010term effects of e\u2010cigarette inhalation on cellular and inflammatory responses within the mouse laryngeal glottic and subglottic regions after exposure to pod\u2010based devices (JUUL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL6\/J mice (8\u20139\u2009weeks) were assigned to control (<jats:italic>n<\/jats:italic>\u2009=\u20099), JUUL flavors Mint (JMi; <jats:italic>n<\/jats:italic>\u2009=\u200910) or Mango (JMa; <jats:italic>n<\/jats:italic>\u2009=\u200910). JUUL mice were exposed to 2\u2009h\/day for 1, 5, and 10\u2009days using the inExpose inhalation system. Control mice were in room air. Vocal fold (VF) epithelial thickness, cell proliferation, subglandular area and composition, inflammatory cell infiltration, and surface topography were evaluated in the harvested larynges. Mouse body weight and urinary nicotine biomarkers were also measured. Chemical analysis of JUUL aerosols was conducted using selective ion flow tube mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>JUUL\u2010exposed mice had reduced body weight after day 5. Urinary nicotine biomarker levels indicated successful JUUL exposure and metabolism. Quantitative analysis of JUUL aerosol indicated that chemical constituents differ between JMi and JMa flavors. VF epithelial thickness, cellular proliferation, glandular area, and surface topography remained unchanged after JUUL exposures. Acidic mucus content increased after 1 day of JMi exposure. VF macrophage and T\u2010cell levels slightly increased after 10\u2009days of JMi exposures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Short\u2010term e\u2010cigarette exposures cause minimal flavor\u2010 and region\u2010specific cellular and inflammatory changes in the mouse larynx. This work provides a foundation for long\u2010term studies to determine if these responses are altered with multiple e\u2010cigarette components and concentrations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A <jats:italic>Laryngoscope<\/jats:italic>, 134:1316\u20131326, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31043","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T13:00:14Z","timestamp":1694523614000},"page":"1316-1326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of Short\u2010term Electronic(e)\u2010Cigarette Aerosol Exposure in the Mouse Larynx"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0377-748X","authenticated-orcid":false,"given":"Meena","family":"Easwaran","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head &amp; Neck Surgery, School of Medicine Stanford University  Stanford California U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe Santa","family":"Maria","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head &amp; Neck Surgery, School of Medicine Stanford University  Stanford California U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua D.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head &amp; Neck Surgery, School of Medicine Stanford University  Stanford California U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine Stanford University  Stanford California U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine Stanford University  Stanford California U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Soo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head &amp; 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