{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:20:53Z","timestamp":1792624853958,"version":"4.5.0"},"reference-count":67,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T00:00:00Z","timestamp":1715212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01DC018060\u20101"],"award-info":[{"award-number":["1R01DC018060\u20101"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives\/Hypothesis<\/jats:title>\n                    <jats:p>Recurrent laryngeal nerve injury diagnosed as idiopathic or due to short\u2010term surgery\u2010related intubation exhibits a higher incidence of left\u2010sided paralysis. While this is often attributed to nerve length, it is hypothesized there are asymmetric differences in the expression of genes related to neuromuscular function that may impact reinnervation and contribute to this laterality phenomenon. To test this hypothesis, this study analyzes the transcriptome profiles of the intrinsic laryngeal muscles (ILMs), comparing gene expression in the left versus right, with particular attention to genetic pathways associated with neuromuscular function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Laboratory experiment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RNA was extracted from the left and right sides of the rat posterior cricoarytenoid (PCA), lateral thyroarytenoid (LTA), and medial thyroarytenoid (MTA), respectively. After high\u2010throughput RNA\u2010Sequencing, 88 samples were organized into 12 datasets according to their age (P15\/adult), sex (male\/female), and muscle type (PCA\/LTA\/MTA). A comprehensive bioinformatics analysis was conducted to compare the left\u2010right ILMs across different conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 774 differentially expressed genes were identified across the 12 experimental groups, revealing age, sex, and muscle\u2010specific differences between the left versus right ILMs. Enrichment analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways implicated several genes with a left\u2010right laryngeal muscle asymmetry. These genes are associated with neuronal and muscular physiology, immune\/inflammatory response, and hormone control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Bioinformatics analysis confirmed divergent transcriptome profiles between the left\u2010right ILMs. This preliminary study identifies putative gene targets that will characterize ILM laterality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>\n                      N\/A\n                      <jats:italic>Laryngoscope<\/jats:italic>\n                      , 134:3741\u20133753, 2024\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.31487","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T05:04:52Z","timestamp":1715231092000},"page":"3741-3753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptome Analysis of Left Versus Right Intrinsic Laryngeal Muscles Associated with Innervation"],"prefix":"10.1002","volume":"134","author":[{"given":"Angela M.","family":"Kemfack","sequence":"first","affiliation":[{"name":"The Center for Voice and Swallowing, Department of Otolaryngology\u2010Head &amp; Neck Surgery Columbia University Irving Medical Center  New York New York U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3628-030X","authenticated-orcid":false,"given":"Ignacio","family":"Hernandez\u2010Morato","sequence":"additional","affiliation":[{"name":"The Center for Voice and Swallowing, Department of 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