{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:16:57Z","timestamp":1780525017171,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL157039"],"award-info":[{"award-number":["R01HL157039"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Investigate the impact of Surgery\u2010induced stress (SIS) on the normal airway repair process after airway reconstruction using a mouse microsurgery model, mass spectrometry (MS), and bioinformatic analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tracheal tissue from non\u2010surgical (<jats:italic>N<\/jats:italic>\u2009=\u20093) and syngeneic tracheal grafts at 3\u2009months post\u2010replacement (<jats:italic>N<\/jats:italic>\u2009=\u20093) were assessed using mass spectrometry. Statistical analysis was done using MASCOT via Proteome Discoverer\u2122. Proteins were categorized into total, dysregulated, suppressed, and evoked proteins in response to SIS. Dysregulated proteins were identified using cut\u2010off values of \u22121 &lt;log<jats:sub>2<\/jats:sub>FoldChange &gt;1 and <jats:italic>t<\/jats:italic>\u2010test (<jats:italic>p<\/jats:italic> value &lt;0.05). Enriched pathways were determined using STRING and Metascape.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At the three\u2010month post\u2010operation mark, we noted a significant increase in submucosal cellular infiltration (14343\u2009\u00b1\u20091286 cells\/mm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009=\u20090.0003), despite reduced overall thickness (30\u2009\u00b1\u20093\u2009\u03bcm, <jats:italic>p<\/jats:italic>\u2009=\u20090.01), compared to Native (4578\u2009\u00b1\u2009723 cells\/mm<jats:sup>2<\/jats:sup>; 42\u2009\u00b1\u20096\u2009\u03bcm). Matrisome composition remained preserved, with proteomic analysis identifying 193 commonly abundant proteins, encompassing 7.2% collagens, 34.2% Extracellular matrix (ECM) glycoproteins, 6.2% proteoglycans, 33.2% ECM regulators, 14.5% Extracellular matrix\u2010affiliated, and 4.7% secreted factors. Additionally, our analysis unveiled a unique proteomic signature of 217 \u201cSurgery\u2010evoked proteins\u201d associated with SIS, revealing intricate connections among neutrophils, ECM remodeling, and vascularization through matrix metalloproteinase\u20109 interaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrated the impact of SIS on the extracellular matrix, particularly MMP9, after airway reconstruction. The novel identification of MMP9 prompts further investigation into its potential role in repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 134:4052\u20134059, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31501","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T10:06:54Z","timestamp":1715681214000},"page":"4052-4059","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic Analysis of Surgery\u2010induced Stress Post\u2010Tracheal Transplantation Highlights Changes in Matrisome"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6729-829X","authenticated-orcid":false,"given":"Jazmin","family":"Calyeca","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"Center for 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 Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9849-6881","authenticated-orcid":false,"given":"Lumei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine Abigail Wexner Research Institute at Nationwide Children's Hospital  Columbus Ohio U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1448-5873","authenticated-orcid":false,"given":"Tendy","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"Center for Regenerative Medicine Abigail Wexner Research Institute at Nationwide Children's Hospital  Columbus Ohio U.S.A."},{"name":"The Ohio State University College of Medicine  Columbus Ohio U.S.A."},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery The Ohio State Wexner Medical Center  Columbus Ohio 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