{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:49:39Z","timestamp":1789390179697,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["5R01DC019632\u201003"],"award-info":[{"award-number":["5R01DC019632\u201003"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Vocal fold paralysis impairs quality of life, and no curative injectable therapy exists. We evaluated injection of a novel in situ polymerizing (scaffold\u2010forming) collagen in the presence and absence of muscle\u2010derived motor\u2010endplate expressing cells (MEEs) to promote medialization and recurrent laryngeal nerve (RLN) regeneration in a porcine model of unilateral vocal fold paralysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twelve Yucatan minipigs underwent right RLN transection. Autologous muscle progenitor cells were isolated from muscle biopsies, differentiated, and induced to MEEs. Three weeks after RLN injury, animals received injections of collagen, collagen containing MEEs, or saline into the paralyzed right vocal fold. Stimulated laryngeal electromyography and acoustic vocalization were used for function assessments. Larynges were harvested and underwent histologic, gene expression, and further quantitative analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Injections were well\u2010tolerated, with the collagen scaffold showing immunotolerance and collagen\u2010encapsulated MEEs remaining viable. Collagen\u2010treated paralyzed vocal folds showed increased laryngeal adductor muscle volumes relative to that of the uninjured side, with those receiving MEEs and collagen showing the highest volumes. Muscles injected with MEEs and collagen demonstrated increased expression of select neurotrophic (\n                      <jats:italic>BDNF<\/jats:italic>\n                      and\n                      <jats:italic>NTN1<\/jats:italic>\n                      ), motor\u2010endplate (\n                      <jats:italic>DOK7<\/jats:italic>\n                      ,\n                      <jats:italic>CHRNA1<\/jats:italic>\n                      , and\n                      <jats:italic>MUSK<\/jats:italic>\n                      ), and myogenic (\n                      <jats:italic>MYOG<\/jats:italic>\n                      and\n                      <jats:italic>MYOD<\/jats:italic>\n                      ) related genes relative to saline controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In a porcine model of unilateral vocal fold paralysis, injection of in situ polymerizing collagen in the absence and presence of MEEs enhanced laryngeal adductor muscle volume, modulated expression of neurotrophic and myogenic factors, and avoided adverse material\u2010mediated immune responses. Further study is needed to determine long\u2010term functional outcomes with this novel therapeutic approach.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>\n                      NA\n                      <jats:italic>Laryngoscope<\/jats:italic>\n                      , 134:4988\u20134997, 2024\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.31642","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T06:20:52Z","timestamp":1721110852000},"page":"4988-4997","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Scaffold\u2010Forming Collagen and Motor\u2010Endplate Expressing Muscle Cells for Porcine Laryngoplasty"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-9394","authenticated-orcid":false,"given":"Elizabeth O.","family":"Shay","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4700-5947","authenticated-orcid":false,"given":"Rachel A.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-7987","authenticated-orcid":false,"given":"Lujuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6629-4895","authenticated-orcid":false,"given":"Samuel L.","family":"Kaefer","sequence":"additional","affiliation":[{"name":"School of Medicine Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy","family":"Wesson","sequence":"additional","affiliation":[{"name":"School of Medicine Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2262-8168","authenticated-orcid":false,"given":"Abigail","family":"Cox","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry L.","family":"Voytik\u2010Harbin","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey","family":"Halum","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e3180de4d49"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhn.2001.117714"},{"key":"e_1_2_7_4_1","series-title":"StatPearl","volume-title":"Unilateral Vocal Fold Paralysis","author":"Singh JM","year":"2022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21754"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2012.06.011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599814520999"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599817721688"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24772"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019128"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20760-6"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31803e8c8d"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23519"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26992"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27575"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30868"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2003.12.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40136-021-00331-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21431"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21537"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3BM00091E"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-81771-x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00073.2018"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00066.2020"},{"key":"e_1_2_7_25_1","first-page":"377","article-title":"Impact of needle selection on survival of muscle\u2010derived cells when used for laryngeal injections","volume":"14","author":"Awonusi O","year":"2023","journal-title":"J Cell Sci Ther"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29282"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.110"},{"issue":"22","key":"e_1_2_7_28_1","first-page":"62","article-title":"Eliciting and characterizing porcine vocalizations: when pigs fly","volume":"0892","author":"Zhang L","year":"2022","journal-title":"J Voice"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215119002494"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215120002558"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-020-06291-2"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2010.03.001"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.31642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T07:08:14Z","timestamp":1731568094000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.31642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,16]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/lary.31642"],"URL":"https:\/\/doi.org\/10.1002\/lary.31642","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,16]]},"assertion":[{"value":"2024-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}