{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T13:28:40Z","timestamp":1777037320314,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL157039"],"award-info":[{"award-number":["R01HL157039"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>A critical barrier to successful tracheal transplantation is poor vascularization. Despite its importance, little is known about microvascular regeneration in tissue\u2010engineered grafts. We have demonstrated that partially decellularized tracheal grafts (PDTG) support neotissue formation including new submucosal microvasculature (CD31+). However, the perfusion of this neovasculature is unknown. In this study, we used a mouse model of tracheal replacement to measure the microvascular regeneration and perfusion of PDTG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PDTG and syngeneic tracheal grafts (STG, surgical control) (<jats:italic>n<\/jats:italic>\u2009=\u20095 for each group) were orthotopically transplanted into C5BL\/6\u2009J mice. We quantified vascularity of STG and PDTG samples at 1 and 3\u2009months with conventional histology (<jats:italic>N<\/jats:italic>\u2009=\u20093\u2009~\u200910\/group). At 1, 3, and 6\u2009months, animals were injected with fluorescein isothiocyanate (FITC) tomato lectin into the left ventricle. After perfusion, tracheas were fixed, harvested, mounted, stained for CD31 expression, and imaged with resonant scanning confocal microscopy. Percent CD31+, FITC area was compared between groups and endpoints compared with native trachea. Microvascular intersections were quantified using Sholl analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Functional microvasculature was seen in both groups. Although percent vascularization (CD31) in PDTG was restored by 3\u2009months, microvascular pattern in PDTG displayed a unique morphology compared with control. Surgery alone appeared to globally change microvascular pattern and perfusion. PDTG demonstrated equivalent perfusion to surgical control by 6\u2009months. Sholl analysis revealed a reduction of microvessel intersectionality that persisted in PDTG and was not seen in surgical or native controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PDTG exhibited microvascular regeneration. Perfusion was present in PDTG, improved, and persisted over long\u2010term time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 135:614\u2013621, 2025<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31801","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T10:10:36Z","timestamp":1728123036000},"page":"614-621","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Microvascular Regeneration and Perfusion of Partially Decellularized Tracheal Grafts"],"prefix":"10.1002","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1238-2953","authenticated-orcid":false,"given":"Ada C.","family":"Sher","sequence":"first","affiliation":[{"name":"The Ohio State University College of Medicine  Columbus Ohio USA"},{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9849-6881","authenticated-orcid":false,"given":"Lumei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melwan","family":"Izem","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6729-829X","authenticated-orcid":false,"given":"Jazmin","family":"Calyeca","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"},{"name":"Department of Otolaryngology\u2010Head &amp; Neck Surgery Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8686-8917","authenticated-orcid":false,"given":"Molly O.","family":"Meeker","sequence":"additional","affiliation":[{"name":"The Ohio State University College of Medicine  Columbus Ohio USA"},{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1448-5873","authenticated-orcid":false,"given":"Tendy","family":"Chiang","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute Nationwide Children's Hospital  Columbus Ohio USA"},{"name":"Department of Otolaryngology\u2010Head &amp; Neck Surgery Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000004509"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2072-1439.2016.01.85"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32311"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10525"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-023-00312-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.11.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/20417314211017417"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.1.9607066"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-014-1301-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3791\/55789"},{"issue":"4","key":"e_1_2_6_12_1","first-page":"387","article-title":"Dendritic organization in the neurons of the visual and motor cortices of the cat","volume":"87","author":"Sholl DA","year":"1953","journal-title":"J Anat"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8080116"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599819844754"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ICVTS\/IVX442"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/428138a"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1673-5374.2013.09.010"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6734"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-022-00447-8"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310842111"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.ra120.012590"},{"key":"e_1_2_6_22_1","volume-title":"Angiogenesis","author":"Aldair T","year":"2010"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.121.057709"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.22.7.076011"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevBiomedEng.2013008077"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1985.tb00081.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.03.002"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.316851"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.31801","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T06:07:48Z","timestamp":1736748468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.31801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,5]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/lary.31801"],"URL":"https:\/\/doi.org\/10.1002\/lary.31801","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,5]]},"assertion":[{"value":"2024-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}