{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T02:00:06Z","timestamp":1783994406330,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["5R01DC014070\u201008"],"award-info":[{"award-number":["5R01DC014070\u201008"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["5R01DC019632\u201002"],"award-info":[{"award-number":["5R01DC019632\u201002"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Partial laryngectomies result in voice, swallowing, and airway impairment for thousands of patients in the United States each year. Treatment options for dynamic restoration of laryngeal function are limited. Thus, there is a need for new reconstructive approaches. Here, we evaluated early (4\u2009week) outcomes of multi\u2010layered mucosal\u2010myochondral (MMC) implants when used to restore laryngeal form and function after hemilaryngectomy in a porcine model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six Yucatan minipigs underwent transmural hemilaryngectomies followed by reconstruction with customized MMC implants aiming to provide site\u2010appropriate localization of regenerated laryngeal tissues, while supporting laryngeal function. All implants were fabricated from polymeric collagen, with a subset of muscle and cartilage implants containing motor endplate\u2010expressing muscle progenitor cells or cartilage\u2010like cells differentiated from adipose stem cells, respectively. Vocalization and laryngeal electromyography (L\u2010EMG) measurements with nerve conduction studies were performed post\u2010operatively and compared with baseline along with gross and histological analyses of the healing response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All animals (<jats:italic>n<\/jats:italic>\u2009=\u20096) survived and maintained airway patency, safe swallowing, and phonation, without the use of tracheostomy and\/or gastrostomy tubes. Histological evaluation indicated no adverse tissue reaction or implant degradation, showing progressive regenerative remodeling with mucosa reformation and ingrowth of new muscle and cartilage. Preliminary L\u2010EMG suggested weak but detectable motor unit action potentials. Although vocalization duration, frequency, and intensity decreased post\u2010operatively, all animals retained vocal capacity and parameter recovery was evident over the study duration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Engineered collagen polymeric implants in the presence or absence of autologous cell populations may serve as a feasible reconstructive option to restore dynamic function after hemilaryngectomy. Long\u2010term follow\u2010up is needed to further assess functional outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 135:769\u2013778, 2025<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31827","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T06:19:34Z","timestamp":1731651574000},"page":"769-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multi\u2010Layered Implant Approach for Hemilaryngectomy Reconstruction in a Porcine Model"],"prefix":"10.1002","volume":"135","author":[{"given":"Troy","family":"Wesson","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4700-5947","authenticated-orcid":false,"given":"Rachel A.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-7987","authenticated-orcid":false,"given":"Lujuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Brookes","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6629-4895","authenticated-orcid":false,"given":"Sam","family":"Kaefer","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Finnegan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8644-9735","authenticated-orcid":false,"given":"Haley","family":"Calcagno","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent J.","family":"Campiti","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry","family":"Voytik\u2010Harbin","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-4403","authenticated-orcid":false,"given":"Stacey","family":"Halum","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Indiana University School of Medicine  Indianapolis Indiana U.S.A."},{"name":"Department of Speech, Language, and Hearing Sciences Purdue University  West Lafayette Indiana 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