{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:28:35Z","timestamp":1792787315625,"version":"4.5.0"},"reference-count":10,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Unilateral thin clear rhinorrhea (UTCR) may represent a variety of pathologies including cerebrospinal fluid (CSF) rhinorrhea. Beta\u20102 transferrin (B2Tf) gel electrophoresis (GE) has become the preferred testing modality due to reportedly high sensitivity (87%\u2013100%) and specificity (71%\u2013100%). However, there have been relatively few studies assessing its diagnostic accuracy. The purpose of this single\u2010institution study was to determine the accuracy of B2Tf GE in detecting CSF rhinorrhea.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A single\u2010center retrospective review was conducted from 2016 and 2024 for all patients who presented with UTCR and underwent B2Tf GE. Institutional review board approval was obtained. The gold standard for diagnostic confirmation of true and false positives (TP, FP) as well as false negatives (FN) was endoscopic exploration. The gold standard for true negative (TN) was response to medical therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 105 patients underwent 149 B2Tf GE tests. 40 (38.1%) patients were diagnosed with CSF rhinorrhea. Of the 149 B2\u2010Tf GE tests, there were 51 TPs, 72 TNs, 20 FPs, and 6 FNs yielding 89.5% sensitivity, 78.3% specificity, 71.8% positive predictive value, and 92.3% negative predictive value, respectively. Of the false results the most common causes for error were purulent sinusitis (<jats:italic>n<\/jats:italic>\u2009=\u20096, 23.1%), possible mucous contamination from nose\u2010blowing during collection (<jats:italic>n<\/jats:italic>\u2009=\u20093, 11.5%), patient collection error (<jats:italic>n<\/jats:italic>\u2009=\u20093, 11.5%), and blood contamination (<jats:italic>n<\/jats:italic>\u2009=\u20091, 3.8%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although these single\u2010institutional data demonstrate test accuracy within ranges previously reported in the literature, they also demonstrate diagnostic limitations. Future studies should explore reasons for erroneous B2Tf GE results and how these may change clinical decision\u2010making.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>IV <jats:italic>Laryngoscope<\/jats:italic>, 2024<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31845","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T09:38:38Z","timestamp":1728898718000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Diagnostic Accuracy of Beta\u20102 Transferrin Gel Electrophoresis for Detecting Cerebrospinal Fluid Rhinorrhea"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3727-8287","authenticated-orcid":false,"given":"Jacob G.","family":"Eide","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5877-634X","authenticated-orcid":false,"given":"William","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussein","family":"Mackie","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Pathology Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0627-0349","authenticated-orcid":false,"given":"Amrita","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karam","family":"Asmaro","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Robin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Rock","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7377-4782","authenticated-orcid":false,"given":"John R.","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Henry Ford Health  Detroit Michigan U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22704"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2008.01.005"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21637"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24160"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-1740622"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.23293"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30801"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459989310900514"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.10.1178"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.23293"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.31845","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T09:38:39Z","timestamp":1728898719000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.31845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,14]]},"references-count":10,"alternative-id":["10.1002\/lary.31845"],"URL":"https:\/\/doi.org\/10.1002\/lary.31845","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,14]]},"assertion":[{"value":"2024-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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