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This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air\/liquid interface and subjected to RNA\u2010seq analysis, RT\u2010qPCR, immunoblotting, and immunostaining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted <jats:italic>p<\/jats:italic>\u2010value, was prostaglandin E receptor 2 (<jats:italic>PTGER2<\/jats:italic>). The upregulation of <jats:italic>PTGER2<\/jats:italic> was confirmed by RT\u2010qPCR and immunoblot. The presence of the EP2 receptor, encoded by the <jats:italic>PTGER2<\/jats:italic> gene, was confirmed by immunocytochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>PTGER2<\/jats:italic> is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin\u2010exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 135:S1\u2013S8, 2025<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.31868","type":"journal-article","created":{"date-parts":[[2024,11,2]],"date-time":"2024-11-02T08:10:18Z","timestamp":1730535018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prostaglandin E Receptor 2 (<scp>EP2<\/scp>) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium"],"prefix":"10.1002","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5673-3279","authenticated-orcid":false,"given":"Prestina","family":"Smith\u2010Davidson","sequence":"first","affiliation":[{"name":"Sinonasal and Olfaction Program, National Institute on 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