{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T05:03:57Z","timestamp":1780031037229,"version":"3.53.1"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["K23DC020988\u201002"],"award-info":[{"award-number":["K23DC020988\u201002"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC018567\u201004"],"award-info":[{"award-number":["R01DC018567\u201004"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R56DC020736\u201007"],"award-info":[{"award-number":["R56DC020736\u201007"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL146401\u201005"],"award-info":[{"award-number":["R01HL146401\u201005"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Idiopathic subglottic stenosis (iSGS) is a progressive fibrotic condition of the subglottis that presents in women of northern European descent. Endoscopic dilation is a common surgical approach to management of iSGS. The surgery\u2010free interval, or the time between endoscopic dilation procedures is considered an indicator of disease severity. Variations in surgery\u2010free intervals among iSGS patients underscore the necessity for prognostic biomarkers. The objective of this study was to explore serum levels of the damage\u2010associated molecular pattern S100A8\/A9 as a prognostic biomarker in iSGS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum from 20 iSGS patients and eight healthy controls was collected and S100A8\/A9 levels were quantified using an ELISA. Patient data, including demographics and surgery\u2010free intervals, were obtained from medical records. Serum S100A8\/A9 levels were compared to surgery\u2010free intervals. S100A8\/A9 was also assessed using gene expression and immunofluorescence in iSGS specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      S100A8\/A9 was significantly elevated (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0413) in the serum of iSGS patients compared to controls (312.75 vs. 181.49\u2009ng\/mL). Linear regression analysis revealed a correlation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.009) between S100A8\/A9 levels and endoscopic surgery\u2010free interval. S100A8\/A9 was significantly elevated (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0011) in patients with surgery\u2010free intervals less than 1\u2009year (455.2\u2009\u00b1\u200960.45\u2009ng\/mL;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) compared to patients with intervals over 1\u2009year (292.5.93\u2009\u00b1\u2009162.4;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>S100A8\/A9 is increased in the serum and tissue of patients with iSGS. In this cohort of iSGS patients, serum S100A8\/A9 was associated with surgery\u2010free intervals, potentially representing a prognostic biomarker. Further research within a larger cohort is needed to confirm these findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>\n                      3\n                      <jats:italic>Laryngoscope<\/jats:italic>\n                      , 135:1724\u20131731, 2025\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.31934","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T04:45:57Z","timestamp":1732682757000},"page":"1724-1731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serum\n                    <scp>S100A8<\/scp>\n                    \/\n                    <scp>A9<\/scp>\n                    Correlates to Surgery\u2010Free Interval in Idiopathic Subglottic Stenosis"],"prefix":"10.1002","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9319-5761","authenticated-orcid":false,"given":"Laura M.","family":"Mafla","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1004-7606","authenticated-orcid":false,"given":"Raymond J.","family":"So","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Abd\u2010Elazem","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel L.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5357-8776","authenticated-orcid":false,"given":"Yee","family":"Chan\u2010Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Lilly","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8619-7312","authenticated-orcid":false,"given":"Ioan A.","family":"Lina","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-1305","authenticated-orcid":false,"given":"Alexander H.","family":"Gelbard","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Vanderbilt University School of Medicine  Nashville Tennessee U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8471-5449","authenticated-orcid":false,"given":"Alexander T.","family":"Hillel","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9255-7265","authenticated-orcid":false,"given":"Kevin M.","family":"Motz","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Johns Hopkins University School of Medicine  Baltimore Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24956"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26098"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599817706930"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30951"},{"issue":"5","key":"e_1_2_6_6_1","first-page":"1402","article-title":"Idiopathic subglottic stenosis","volume":"76","author":"Brandenburg 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