{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:59:06Z","timestamp":1790380746626,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The primary objective of this pilot study was to compare vocal fold morphology between aged and non\u2010aged adult rat vocal folds. Secondarily, we set out to quantify neurophysiologic and histologic differences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four\u2010month (non\u2010aged) and 21\u2010month (aged) adult Fischer 344xBrown Norway (F344xBN) hybrid rats were used. Vocal fold measurements including bowing index (BI), abduction angle, and normalized vocal fold width (NVFW) were quantified. Laryngeal evoked response studies were performed by stimulating the recurrent laryngeal nerve (RLN) and recording from the thyro\u2010arytenoid (TA) muscle. Histology was performed on laryngeal muscles and the RLN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ten non\u2010aged (4\u2010month) vs. 10 aged (21\u2010month) rat hemi\u2010laryngeal preparations were included. The mean BI was higher in the aged group compared to the non\u2010aged group, 5.02\u2009\u00b1\u20091.05 vs. 4.12\u2009\u00b1\u20091.05\u2009units, respectively, and this difference approached statistical significance (difference 0.89\u2009units, 95% CI: \u22120.10\u20131.80\u2009units, <jats:italic>p<\/jats:italic>\u2009=\u20090.07). CMAP latency was significantly higher in the aged vs. non\u2010aged group, 0.90\u2009\u00b1\u20090.05\u2009ms compared to 0.84\u2009\u00b1\u20090.06\u2009ms, respectively (difference 0.06\u2009ms, 95% CI: 0.01\u20130.11\u2009ms, <jats:italic>p<\/jats:italic>\u2009=\u20090.02). Mean % myelin between the non\u2010aged and aged rats was 72.12%\u2009\u00b1\u20098.55% and 65.41%\u2009\u00b1\u20095.38%, respectively, mean difference \u22126.71% (95% CI: \u221213.72%\u20130.31%, <jats:italic>p<\/jats:italic>\u2009=\u20090.060). Mean cross\u2010sectional area (CSA) of TA myofibers was significantly increased in the aged rats 81.6\u2009\u03bcm<jats:sup>2<\/jats:sup> (95% CI: 6.9\u2013156.3\u2009\u03bcm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009=\u20090.034).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Laryngeal evoked responses showed a significant increase in the CMAP latency in aged rats compared to non\u2010aged rats. There was an increase in BI and a decrease in myelination in the aged rat that approached statistical significance. Future adequately powered studies are needed to quantify the difference in endoscopic measures, neurophysiology, and histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.32212","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T08:14:39Z","timestamp":1745396079000},"page":"3257-3264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quantification of Vocal Fold Atrophy in an Aging Rat Model"],"prefix":"10.1002","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8933-5477","authenticated-orcid":false,"given":"Neel K.","family":"Bhatt","sequence":"first","affiliation":[{"name":"Division of Laryngology, Department of Otolaryngology\u2014Head and Neck Surgery University of Washington School of Medicine  Seattle Washington 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