{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T16:01:36Z","timestamp":1771084896091,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2019JJ50967"],"award-info":[{"award-number":["2019JJ50967"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ30821"],"award-info":[{"award-number":["2022JJ30821"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To elucidate the mechanism underlying the involvement of miR\u2010200b\u20103p in the bone destruction in cholesteatoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Expression levels of miR\u2010200b\u20103p, parathyroid hormone\u2010related protein (PTHrP), and receptor activator for nuclear factor\u2010kappa B ligand (RANKL) in cholesteatoma and normal skin of the external auditory canal were examined. HaCaT cells were transfected with miR\u2010200b\u20103p mimic, miR\u2010200b\u20103p mimic NC, miR\u2010200b\u20103p inhibitor, and miR\u2010200b\u20103p inhibitor NC. Meanwhile, RAW264.7 cells were cultured with RANKL and PTHrP\u2009+\u2009RANKL combined intervention. A bone resorption lacunae experiment was performed to assess the bone absorption function of the osteoclasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The expression level of miR\u2010200b\u20103p significantly decreased in cholesteatoma and was obviously correlated with the degree of bone destruction. However, the expression levels of PTHrP and RANKL significantly increased. An upregulation of miR\u2010200b\u20103p expression in the miR\u2010200b\u20103p mimic group of HaCaT cells was observed, accompanied by a significant downregulation of PTHrP expression. The miR\u2010200b\u20103p inhibitor group showed downregulated miR\u2010200b\u20103p expression and significantly upregulated PTHrP expression, suggesting a negative regulatory effect of miR\u2010200b\u20103p on PTHrP expression. Scanning electron microscopic analysis of the bone resorption lacunae revealed a significant increase in the number and size of bone resorption lacunae in the PTHrP\u2009+\u2009RANKL combined intervention group compared to those in the RANKL intervention group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MiR\u2010200b\u20103p was underexpressed in cholesteatoma, resulting in the upregulation of PTHrP secreted by keratinocytes, activation of the downstream RANKL pathway, and promotion of differentiation of monocyte macrophages in the cholesteatoma perimatrix into osteoclasts, thus contributing to bone destruction in cholesteatoma.<\/jats:p>\n                    <jats:p>\n                      <jats:bold>Level of Evidence:<\/jats:bold>\n                      N\/A.\n                    <\/jats:p>\n                  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