{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:49:38Z","timestamp":1789390178889,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Unilateral vocal fold paralysis (UVFP) due to recurrent laryngeal nerve injury (RLN) is a major cause of voice disorders. We have recently identified three biomolecules (agrin, acetylcholine, and neuregulin) with the potential to promote reinnervation after RLN injury. This study aimed to determine if a gelatin injectable with the reinnervating biomolecules will induce site\u2010specific amplification of neurotrophic factor release and reinnervation after RLN injury in unilateral vocal fold paralysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      C57BL\/6 mice underwent RLN transection with the following treatment allocations: saline control (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200916), biomolecule cocktail only (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200916), and biomolecule\u2010infused gelatin (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200916). All injectables were delivered into the denervated thyroarytenoid muscle. Assessment of glottic function was determined via laryngeal electromyography (L\u2010EMG) and stimulated video laryngoscopy post\u2010RLN transection on days 7 and 28. Histopathology analysis via immunohistochemistry (IHC) and genetic analysis via quantitative polymerase chain reaction (qPCR) were utilized to characterize reinnervation and gene expression changes within the harvested larynges.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both biomolecule treatment groups had enhanced reinnervation of the adductor complex, as indicated by higher stimulated L\u2010EMG area under the curve (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), adduction during stimulation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.002), midline resting position (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.002), and the presence of innervated neuromuscular junctions on IHC. qPCR results suggest both biomolecule treatments resulted in elevated neurotrophic and angiogenic factor expression from the injected muscle.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Injectable biomolecule\u2010infused gelatin may serve as a novel long\u2010term therapeutic for glottic functional restoration by redirecting existing mechanisms of synkinesis via site\u2010specific neurotrophic factor release.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>N\/A.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.32459","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T12:40:19Z","timestamp":1757940019000},"page":"4781-4792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel Biomolecule\u2010Infused Gelatin Injectable for Treatment of Recurrent Laryngeal Nerve Injury"],"prefix":"10.1002","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1447-6096","authenticated-orcid":false,"given":"Ananya","family":"Tadikonda","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-6943","authenticated-orcid":false,"given":"Sunjay","family":"Anekal","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena W.","family":"Chen","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6501-1566","authenticated-orcid":false,"given":"Gabriel","family":"Sobczak","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy","family":"Wesson","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmilya","family":"Jackson","sequence":"additional","affiliation":[{"name":"Meharry Medical College  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa A.","family":"Egan","sequence":"additional","affiliation":[{"name":"Davidson School of Chemical Engineering Purdue University  West\u00a0Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie C.","family":"Liu","sequence":"additional","affiliation":[{"name":"Davidson School of Chemical Engineering Purdue University  West\u00a0Lafayette Indiana USA"},{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3750-8428","authenticated-orcid":false,"given":"Patrick R.","family":"Finnegan","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey","family":"Halum","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.34078"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2007.10.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2019.3072"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0145561320965212"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-017-4758-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348941011900601"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-022-07437-0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40136-021-00331-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27684"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599818817762"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2010.09.031"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599817721688"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31816c438e"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23519"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31803e8c8d"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30868"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26992"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30885"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.31642"},{"key":"e_1_2_9_21_1","first-page":"16","volume-title":"Primed 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