{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:30:59Z","timestamp":1791469859698,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC020176"],"award-info":[{"award-number":["R01 DC020176"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The laryngeal epithelium contains a basal cell (BC) layer. The BC layer is a reservoir of progenitor cells necessary for the formation and regeneration of the epithelium. The epithelium is susceptible to the deposition of inhaled irritants such as cigarette smoke (CS) and electronic cigarette (E\u2010Cig) aerosol. The objective of this study was to quantify BC, proliferating cells, and epithelial histologic architecture in the larynx following a 6\u2010week exposure to CS or E\u2010Cig aerosol.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mice (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200912) were assigned to a room\u2010air control, CS\u2010exposed, or an E\u2010Cig\u2010exposed group. Exposures were conducted for 2\u2009h per day, 5\u2009days a week for a total of 6\u2009weeks. Mice were then euthanized and the larynx was evaluated using immunofluorescence and histology. We assessed p63\u2010positive BCs, Ki67\u2010positive proliferating cells, p63 and Ki67 colocalized cells, overall cellularity, and epithelial thickness across the supraglottic, vocal fold, and subglottic regions of the larynx.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following E\u2010Cig exposure, there was a significant reduction in p63\u2010positive BC in the vocal folds as compared to the CS\u2010exposed and control group and epithelial thickness compared to the CS\u2010exposed group. No significant changes were observed in the numbers of BCs, proliferating cells, colocalized cells, or overall cellularity in any of the other laryngeal regions examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>An E\u2010Cig\u2010induced reduction in BCs within the vocal fold epithelium may have implications for decreased regenerative capacity. Overall, our findings highlight distinct epithelial responses to CS and E\u2010Cig exposures and underscore the regional heterogeneity of the laryngeal response to these common inhaled challenges.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70092","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T11:52:04Z","timestamp":1756727524000},"page":"838-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Laryngeal Basal Cell Response to Cigarette Smoke and E\u2010Cigarette Aerosol Exposures"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0377-748X","authenticated-orcid":false,"given":"Meena","family":"Easwaran","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head &amp; Neck Surgery Stanford University School of Medicine  Stanford California 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