{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:41:56Z","timestamp":1781624516071,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI14C1277"],"award-info":[{"award-number":["HI14C1277"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Tracheal stenosis with long lesions poses a challenge for surgical treatment, requiring a bioengineering approach. However, synthetic tracheal scaffolds often fail due to the host immune response against the implant. Nonetheless, the key immune cells in the tracheal environment and their interactions against scaffolds remain poorly understood. In this study, we examined the tracheal immune cell compositions and their dynamics in response to the scaffold implantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used C57BL\/6 mice to develop a model for identifying immune cells. For the scaffold implantation, a circumferential tracheal defect was made, and a poly(\u03b5\u2010caprolactone) (PCL) scaffold was orthotopically implanted. Tracheal cells were isolated using an enzyme digestion protocol, and immune cells and their subsets were identified by flow cytometry on days 1 and 7 post\u2010implantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A circumferential tracheal defect was successfully created in mice, followed by the implantation of a PCL scaffold. Viable immune cells were consistently isolated from the trachea. Diverse populations of innate and adaptive immune cells were recovered, with CD3\u2010B220\u2010myeloid cells constituting the predominant population in normal mouse trachea. Importantly, implantation of the PCL scaffold severely disrupted local immune homeostasis, inducing a strong CD8\u03b1\n                      <jats:sup>\u2212<\/jats:sup>\n                      T cell response, reaching approximately 65% of total immune cells on postoperative day 1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We present findings on immune responses in the trachea, highlighting the immune cells involved in maintaining homeostasis and their disruption following scaffold implantation. These insights could support the development of targeted immunomodulation strategies, thereby improving the integration and clinical application of biomaterials in tracheal reconstruction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>NA<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70139","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T11:35:25Z","timestamp":1758108925000},"page":"694-702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling Immune Responses in the Trachea Following Scaffold Implantation in a Mouse Model"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3644-0289","authenticated-orcid":false,"given":"Jeon","family":"Seong","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Interdisciplinary Program in Stem Cell Biology Seoul National University  Seoul Republic of Korea"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, Dental Research Institute Seoul National University Dental Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haeyoon","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Suk","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0333-6349","authenticated-orcid":false,"given":"Joo\u2010Young","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, Dental Research Institute Seoul National University Dental Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Keun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Interdisciplinary Program in Stem Cell Biology Seoul National University  Seoul Republic of Korea"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Cancer Research Institute Seoul National University  Seoul Republic of Korea"},{"name":"Sensory Organ Research Institute Seoul National University, Medical Research Center  Seoul Republic of\u00a0Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(95)70279-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semtcvs.2009.08.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2013.03.017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sempedsurg.2021.151055"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2003.260"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2004.07.005"},{"key":"e_1_2_9_8_1","article-title":"Tracheal Replacement","volume":"8","author":"Delaere P.","year":"2016","journal-title":"Journal of Thoracic Disease"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02211-2017"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2019.05.050"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13665-022-00286-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052421"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otoeng.2017.01.011"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/children8070589"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.129.1.118"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2022.111299"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00914037.2018.1466139"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32052"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2019.04.039"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2017.07.006"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108553"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.3c00553"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/7\/3\/035015"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00250"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489419826134"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.04.021"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/ac5574"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2024.06.013"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70139","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.70139","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T08:51:09Z","timestamp":1768207869000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.70139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,17]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/lary.70139"],"URL":"https:\/\/doi.org\/10.1002\/lary.70139","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,17]]},"assertion":[{"value":"2025-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}