{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T09:57:10Z","timestamp":1781258230876,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01004336"],"award-info":[{"award-number":["R01004336"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["T32DC009401"],"award-info":[{"award-number":["T32DC009401"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010836","name":"American Laryngological, Rhinological and Otological Society","doi-asserted-by":"publisher","award":["CareerDevelopmentAward"],"award-info":[{"award-number":["CareerDevelopmentAward"]}],"id":[{"id":"10.13039\/100010836","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective(s)<\/jats:title>\n                    <jats:p>Acute laryngeal injury (ALgI) occurs in over 50% of patients after intubation and mechanical ventilation and is associated with significantly worse voice, breathing, and swallowing outcomes. Currently, there are no small animal models for the study of ALgI and its progression to glottic stenosis. The objective of this study was to develop and validate a novel murine model of ALgI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Thirty adult C57BL\/6 mice underwent chemomechanical injury to the posterior glottis, and 16 control mice did not undergo injury. Glottic injury was performed under endoscopic guidance using a bleomycin\u2010dipped wire brush. Mice underwent repeat endoscopy at 14\u2010 or 21\u2010days following injury, and the maximal interarytenoid angle during respiration was quantified using ImageJ to evaluate glottic mobility. Histologic and gene expression analyses were performed on larynges from each group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The interarytenoid angle of injured mice was significantly reduced compared to controls at both 14 (35.3\u00b0 vs. 68.0\u00b0,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.016) and 21\u2009days post\u2010injury (34.5\u00b0 vs. 68.0\u00b0,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). There was a significant increase in posterior glottic thickness in injured compared to control mice at 21 (132.5 vs. 53.9,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) but not 14\u2009days post\u2010injury (90.4 vs. 53.9,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.1535). Trichrome staining and RT\u2010qPCR demonstrated collagen upregulation in the posterior glottis of injured mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Chemomechanical injury to the posterior glottis produces a novel murine model of ALgI. This safe, reliable, and feasible model lays the foundation for future translational study of ALgI and its progression to glottic stenosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>N\/A animal study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70244","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T10:01:26Z","timestamp":1761818486000},"page":"1784-1791","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Murine Model of Acute Laryngeal Injury After Intubation"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9592-932X","authenticated-orcid":false,"given":"Ruth J.","family":"Davis","sequence":"first","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology\u2014Head and Neck Surgery University of Wisconsin\u2010Madison School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Kreuser","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology\u2014Head and Neck Surgery University of Wisconsin\u2010Madison School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadeas","family":"Lunga","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology\u2014Head and Neck Surgery University of Wisconsin\u2010Madison School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan E.","family":"Schaub","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology\u2014Head and Neck Surgery University of Wisconsin\u2010Madison School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-4356","authenticated-orcid":false,"given":"Susan L.","family":"Thibeault","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Otolaryngology\u2014Head and Neck Surgery University of Wisconsin\u2010Madison School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000003368"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000004015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00455-023-10559-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2019.3022"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489414551980"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2020.4517"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29385"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26893"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29026"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27321"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599820978271"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940511400712"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2008.03.007"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198506000-00012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2007.05.019"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.168"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348949810701005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2014.1663"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/LARY.31499"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489414564249"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2015.1136"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599810392894"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14051000"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12040450"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/LARY.31138"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2018.07.012"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_2"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000150701.46377.df"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.28669"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30473"},{"key":"e_1_2_8_33_1","article-title":"Minimally 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