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Most approaches require high\u2010tech visualization and lengthy anesthesia. Intraperitoneal (IP) naphthalene (NAPH) and topical polidocanol (PDOC) are two chemical methods of injuring murine lower airways. Oropharyngeal aspiration (OA) is used for murine lung instillation. We assessed whether these simple low\u2010tech methods could injure and instill mouse VFs, and whether injury increased in\u00a0vivo VF epithelial permeability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mouse VFs were injured using IP NAPH at 200, 250, 300, or 350\u2009mg\/kg, or OA of PDOC at 0.5% or 2% w\/v. Twenty\u2010four hours later, mice received Evans blue (EB) dye OA instillation, then were sacrificed after 30\u2009min. Coronal larynx sections were assessed for VF injury. Permeability was measured by imaging EB autofluorescence and quantifying percent positive area and signal intensity. VFs were immunostained for basal cells (p63), tight junctions (ZO\u20101), and basement membrane (laminin).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>VF injury was 100% with 350\u2009mg\/kg NAPH or high\u2010volume 2% PDOC and 0%\u201340% with other treatments. EB bound VF lamina propria in up to 100% of mice in a volume\u2010dependent manner. Permeability did not differ by injury. Basal cells and tight junctions were decreased in injured VFs. Basement membrane was largely intact.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>High\u2010dose NAPH consistently injures mouse VFs. OA reliably instills mouse VFs. Uninjured murine VF epithelium is highly permeable to low molecular weight dye in\u00a0vivo. Inherent permeability of mouse VFs may facilitate targeted genetic engineering approaches and studies of environmental hazards and drug treatments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>n\/a.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70266","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:24:37Z","timestamp":1765355077000},"page":"1846-1857","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mouse Vocal Fold Permeability In\u00a0Vivo: Effects of Novel Low\u2010Tech Injury and Instillation Methods"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6540-3834","authenticated-orcid":false,"given":"Renee E.","family":"King","sequence":"first","affiliation":[{"name":"McArdle Laboratory for Cancer 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