{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T10:13:23Z","timestamp":1777976003617,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Unilateral vocal fold paralysis (UVFP) due to recurrent laryngeal nerve (RLN) injury is a common cause of dysphonia. No biotherapeutic injectable exists that directs laryngeal reinnervation after RLN injury. Placental\u2010derived connective tissue matrix (pd\u2010CTM) could fill this need, as it contains a plethora of cytokines with potential UVFP therapeutic benefits. This study aimed to identify and quantify the factors in a commercially available pd\u2010CTM (CTM Flow, CTM Biomedical, Lake Worth, Florida) and to study the effects of pd\u2010CTM on vocal fold microenvironment and glottic function in a mouse model of unilateral RLN injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Cytokine expression (ng\/mL) in pd\u2010CTM was characterized using a cytokine array and ELISA. In a separate experiment, C57\/BL6 mice were divided into three groups: uninjured negative controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912), RLN transection with ipsilateral saline thyroarytenoid (TA) injection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200916), and RLN transection with ipsilateral pd\u2010CTM TA injection. Outcomes included laryngeal electromyography (L\u2010EMG) and video laryngoscopy after 7 and 28\u2009days, with larynges then harvested and analyzed via immunohistochemistry (IHC) and qPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      pd\u2010CTM characterization showed moderate\u2010to\u2010high levels of neurotrophic (BDNF, CNTF, GDNF, NTF\u20103), angiogenic (Angiogenin, VEGF\u2010D), tissue remodeling (bFGF, IGF\u20101, HGF, TGF\u2010\u03b23), and anti\u2010inflammatory factors (IL\u201010, IL\u20101R\u03b1). L\u2010EMG demonstrated increased mean normalized area under the curve ratio in pd\u2010CTM treated mice compared to saline treated mice at the 28\u2010day time point indicating reinnervation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). IHC detected innervated neuromuscular junctions 28\u2009days after pd\u2010CTM treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>pd\u2010CTM may be a novel treatment option for patients with UVFP based on the neurotrophic, angiogenic, tissue remodeling, and anti\u2010inflammatory factors present.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>NA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70313","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T09:05:32Z","timestamp":1766394332000},"page":"2220-2231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Placental\u2010Derived Connective Tissue Matrix Mediates Murine Recurrent Laryngeal Nerve Regeneration"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-6943","authenticated-orcid":false,"given":"Sunjay","family":"Anekal","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1447-6096","authenticated-orcid":false,"given":"Ananya","family":"Tadikonda","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6501-1566","authenticated-orcid":false,"given":"Gabriel","family":"Sobczak","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lena W.","family":"Chen","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aditya","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troy","family":"Wesson","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick R.","family":"Finnegan","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacey","family":"Halum","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine (IUSM)  Indianapolis Indiana USA"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery IUSM  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-018-5207-x"},{"key":"e_1_2_10_3_1","volume-title":"StatPearls","author":"Singh J. M.","year":"2024"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2018.0067"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.1125"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-5998(98)80014-4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2022.05.015"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2023.04.010"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/JS9.0000000000001978"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20982"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112417"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-017-0191-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27538"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25739"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1999.19410001.x"},{"key":"e_1_2_10_16_1","first-page":"155","article-title":"The Insulin\u2010Like Growth Factor System: Towards Clinical Applications","volume":"25","author":"Bach L. A.","year":"2004","journal-title":"Clinical Biochemist Reviews"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977199209008871"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.09.010"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(96)00099-9"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-019-0338-1"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599817721688"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.28207"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.27887"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05310.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32292"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes9070316"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23519"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302665"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0738"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34054"},{"key":"e_1_2_10_31_1","article-title":"A Flowable Placental Tissue Matrix Allograft in Lower Extremity Injuries: A Pilot Study","volume":"7","author":"Lullove E.","year":"2015","journal-title":"Cureus"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40634-022-00509-4"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6751456"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/JSM.0000000000000684"},{"key":"e_1_2_10_35_1","first-page":"397","article-title":"Operative Applications of Placental Tissue Matrix in Orthopaedic Sports Injuries: A Review of the Literature","volume":"34","author":"Sultan A. A.","year":"2019","journal-title":"Surgical Technology International"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1328261"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.31642"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2021.102564"},{"key":"e_1_2_10_39_1","article-title":"The Yucatan Minipig: A Reliable Model for Studying Unilateral Vocal Fold Paralysis","volume":"10","author":"Kaefer S. L.","year":"2024","journal-title":"Journal of Voice"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30868"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00204.2014"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.143"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1307447"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2017.01.007"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70313","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.70313","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T09:40:32Z","timestamp":1777974032000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.70313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/lary.70313"],"URL":"https:\/\/doi.org\/10.1002\/lary.70313","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,22]]},"assertion":[{"value":"2025-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}