{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:46:10Z","timestamp":1792053970514,"version":"4.4.0"},"reference-count":57,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA016087"],"award-info":[{"award-number":["P30CA016087"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC021453"],"award-info":[{"award-number":["R01DC021453"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R21DC021796"],"award-info":[{"award-number":["R21DC021796"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To test whether unilateral recurrent laryngeal nerve (RLN) transection elicits side specific transcriptomic responses in rat vocal fold compartments. We compared the medial thyroarytenoid (MTA) muscle and mucosa on the left versus right, accounting for baseline laterality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Unilateral RLN transection was performed in five adult rats (Left RLN: 2\u2010males, 1\u2010female; Right RLN: 1\u2010male, 1\u2010female). 5\u2010weeks post\u2010injury, the medial thyroarytenoid (MTA) muscle and vocal fold mucosa were harvested for RNA sequencing. Differential expression analysis compared Left\u2010denervated versus Right\u2010denervated tissues. A separate control Left\u2010versus\u2010Right analysis (1\u2010male; 1\u2010female) was used to assess baseline laterality and to contextualize TF transcription\u2010factor inferences. TF activity was inferred using perturbation\u2010based signatures, and baseline trends were used to classify overlapping signals as consistent with, opposite to, or distinct from baseline laterality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Left RLN denervation elicited distinct transcriptional responses in both compartments. In mucosa, TFs governing epithelial stability were reduced while regenerative cues were activated, suggesting impaired differentiation and recruitment of progenitor\u2010like programs. In MTA, left\u2010sided injury increased TFs consistent with stress signaling and mitochondrial compensation. TFs involved in epigenetic stability were reduced. Lateralized activity was observed in developmental regulators putatively related to positional memory or differential stress adaptation. Pathway analysis revealed enrichment of skeletal development processes in MTA and immune\/matrix regulation in mucosa, indicating coordinated remodeling across muscle and epithelial compartments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>RLN injury produces side\u2010specific muscle and mucosal changes, with left\u2010sided denervation showing stronger atrophy and stress responses. These results emphasize the importance of laterality and coordinated tissue adaptation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>NA<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70708","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T01:56:38Z","timestamp":1782266198000},"page":"4928-4937","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Transcriptomic Effects of Lateralized\n                    <scp>RLN<\/scp>\n                    Denervation in Rat Vocal Fold"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5362-0866","authenticated-orcid":false,"given":"Gary","family":"Gartling","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery NYU Grossman School of Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3010-5618","authenticated-orcid":false,"given":"Masayoshi","family":"Yoshimatsu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery NYU Grossman School of Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Bing","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery NYU Grossman School of Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan C.","family":"Branski","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery NYU Grossman School of Medicine  New York New York 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