{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:46:27Z","timestamp":1792053987763,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Cell\u2010based outer vocal fold replacement (COVR) consists of human adipose\u2010derived stem cells and is a potential treatment for severe vocal fold injury. Prior rabbit studies demonstrated enhanced wound healing and COVR's safety in pigs. Building on prior work defining pig\u2010specific acoustic parameters, this study evaluates phonatory recovery and establishes quantitative voice assessment frameworks following bilateral cordectomy and COVR implantation in a large animal model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>COVR implantation was performed in eight Yucatan mini\u2010pigs after bilateral vocal fold resection. Functional voice recovery was tracked, with spontaneous vocalizations recorded pre\u2010 and post\u2010surgery for up to 6\u2009months. Pig squeals were extracted using Python, manually verified, and nonrepresentative sounds excluded. Acoustic features including Q50 (median frequency of spectral energy), flux (rate of spectral energy change), F0 (fundamental frequency), jitter (period perturbation), and shimmer (amplitude perturbation) were analyzed using linear mixed\u2010effect models and Wilcoxon rank sum test with Benjamini\u2013Yekutieli correction. After 6\u2009months, larynges were harvested and human cells were sought via fluorescent in\u00a0situ hybridization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Q50 significantly decreased immediately postoperatively (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and returned to preoperative range by 6\u2009months, while F0 and flux changed modestly over time. Human cells persisted in 7 of the 8 subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>COVR implantation improved key nonperiodic acoustic parameters 6\u2009months after extensive laryngeal injury in pigs. Implanted cells were detected after 6\u2009months. Future work includes one\u2010year monitoring to assess long\u2010term voice recovery and extracellular matrix remodeling, artificial intelligence integration for expanded acoustic analysis, and a unilateral injury model comparing control cordectomy alone versus COVR reconstruction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>N\/A.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.70738","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:17:26Z","timestamp":1783948646000},"page":"4957-4968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vocal Outcomes After Cell\u2010Based Outer Vocal Fold Implant in Porcine Bilateral Vocal Fold Resection"],"prefix":"10.1002","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2117-1481","authenticated-orcid":false,"given":"Keer","family":"Zhang","sequence":"first","affiliation":[{"name":"UCLA David Geffen School of Medicine  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia","family":"Crane","sequence":"additional","affiliation":[{"name":"UCLA David Geffen School of Medicine  Los Angeles California USA"},{"name":"UCLA Samueli School of Engineering  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateo Ruiz","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Division of Phoniatrics and Pediatric Audiology at the Department of Otorhinolaryngology Head &amp; Neck Surgery University Hospital Erlangen, Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Klomhaus","sequence":"additional","affiliation":[{"name":"UCLA David Geffen School of Medicine  Los Angeles California USA"},{"name":"Department of Medicine UCLA David Geffen School of Medicine  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yazeed","family":"Alhiyari","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery UCLA David Geffen School of Medicine  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavani","family":"Gowda","sequence":"additional","affiliation":[{"name":"Greater Los Angeles Veterans Affairs Healthcare System  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"D\u00f6llinger","sequence":"additional","affiliation":[{"name":"Division of Phoniatrics and Pediatric Audiology at the Department of Otorhinolaryngology Head &amp; Neck Surgery University Hospital Erlangen, Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4185-2328","authenticated-orcid":false,"given":"Jennifer","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery UCLA David Geffen School of Medicine  Los Angeles California USA"},{"name":"Greater Los Angeles Veterans Affairs Healthcare System  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459989611500521"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2007.12.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2003.06.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25820"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010022\u201002853\u20109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20719"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.929"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30952"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29282"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940111001207"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.31827"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.31642"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0284135"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/app11104489"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000028474"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135414"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2015.10.019"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.70144"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26801"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100686\u20102"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.188839.114"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489418806008"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomimetics7030087"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004050000299"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/s23042293"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.1474440"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2020.01.013"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20755"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/s25134058"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2024.12.008"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70738","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.70738","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.70738","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:10:36Z","timestamp":1792051836000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.70738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,13]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/lary.70738"],"URL":"https:\/\/doi.org\/10.1002\/lary.70738","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,13]]},"assertion":[{"value":"2026-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}