{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:07:48Z","timestamp":1789132068998,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Our previous investigations showed that tissue stem cells in the maculae flavae (a stem cell niche) form colonies in vivo like stem cells in vitro. However, the roles of colony\u2010forming cells in the maculae flavae in vivo have not yet been determined.<\/jats:p><jats:p>This study investigated the metabolism of the colony\u2010forming tissue stem cells in the maculae flavae of the human adult vocal fold.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>Histologic analysis of the human vocal folds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three normal human adult vocal folds were investigated under transmission electron microscopy and light microscopy including immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mitochondrial cristae of the colony\u2010forming cells in the maculae flavae were sparse. Hence, the microstructural features of the mitochondria suggested that their metabolic activity and oxidative phosphorylation were low. Colony\u2010forming cells strongly expressed glucose transporter\u20101 and glycolytic enzymes (hexokinase II, glyceraldehyde\u20103\u2010phosphate dehydrogenase and lactate dehydrogenase A). The colony\u2010forming cells did not express phosphofructokinase\u20101 but did express glucose\u20106\u2010phosphate dehydrogenase indicating the cells relied more on the pentose phosphate pathway. Since the colony\u2010forming cells expressed lactate dehydrogenase A, cells seemed to rely more on anaerobic glycolysis in an anaerobic microenvironment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study is consistent with the hypothesis that the colony\u2010forming tissue stem cells in the maculae flavae of the human adult vocal fold seemed to rely more on anaerobic glycolysis using the pentose phosphate pathway for energy supply in vivo. Microstructural features of the mitochondria and expressed glycolytic enzymes of the colony\u2010forming cells in the maculae flavae suggested that the oxidative phosphorylation activity was low.<\/jats:p><jats:p>In an anaerobic microenvironment in vivo, there is likely a complex cross\u2010talk regarding the metabolism between the colony\u2010forming aggregated cells along the adhesion machinery and chemical signaling pathways, which reduces toxic oxygen species and is favorable to maintaining the stemness and undifferentiated states of the tissue stem cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.550","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T10:00:58Z","timestamp":1616407258000},"page":"283-290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Role of colony\u2010forming tissue stem cells in the macula flava of the human vocal fold in vivo"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5415-0176","authenticated-orcid":false,"given":"Kiminori","family":"Sato","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9307-2331","authenticated-orcid":false,"given":"Shun\u2010ichi","family":"Chitose","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6537-4490","authenticated-orcid":false,"given":"Kiminobu","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7990-7590","authenticated-orcid":false,"given":"Fumihiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2414-7034","authenticated-orcid":false,"given":"Takeharu","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2347-495X","authenticated-orcid":false,"given":"Hirohito","family":"Umeno","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Kurume University School of Medicine  Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348941212101205"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489415578710"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215116001225"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215116008227"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-69194-7_9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-5586-7_12"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.219"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2018.12.002"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.285"},{"key":"e_1_2_9_11_1","unstructured":"SatoK ChitoseS SatoF SatoK OnoT UmenoH.Fine structures of colony\u2010forming tissue stem cells in the macula flava of the human vocal fold in vivo. 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