{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:46:19Z","timestamp":1792442779129,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Betahistine is a partial H1 receptor agonist and a potent H3 receptor antagonist commonly used for the treatment of MD and peripheral vertigo. The aim of this study was to investigate the impact of betahistine on the salt induced cytokine expression profiles of AIED and MD patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood mononuclear cells (PBMCs) were obtained from 24 patients with autoimmune inner ear disease (AIED) or Meniere's disease (MD) during an acute exacerbation of hearing loss. These PBMCs were cultured with 80\u2009mM NaCl or a combination of 80\u2009mM NaCl and betahistine and IL\u20101\u03b2 and IL\u20106 expression were measured by real time PCR and ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In most patients, IL\u20101\u03b2 expression in response to NaCl exceeded the unstimulated condition and this expression was abrogated by the addition of betahistine, which was statistically significant at <jats:italic>p<\/jats:italic>\u2009=\u2009.004. mRNA expression of IL\u20101\u03b2 was not reduced when samples were treated with both salt and betahistine compared to samples treated with salt alone, inferring the mechanism of betahistine\u2010mediated IL\u20101\u03b2 suppression is post\u2010translational. Similarly, IL\u20106 cellular release was augmented with salt exposure and reduced with co\u2010culture of betahistine. Unlike IL\u20101 however, betahistine appeared to reduce IL\u20106 mRNA expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We observe that betahistine abrogates salt\u2010induced IL\u20101\u03b2 expression, suggesting an additional treatment option for AIED and MD patients with inflammatory mediated disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of evidence<\/jats:title><jats:p>Level 4.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.70032","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T11:54:34Z","timestamp":1730980474000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of betahistine on pro\u2010inflammatory cytokine expression in autoimmune inner ear disease and Meniere's disease patients"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-0246","authenticated-orcid":false,"given":"Ilana","family":"Yellin","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Zucker School of Medicine at Hofstra\/Northwell  New Hyde Park New York USA"},{"name":"Feinstein Institute for Medical Research  Manhasset New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shresh","family":"Pathak","sequence":"additional","affiliation":[{"name":"Feinstein Institute for Medical Research  Manhasset New York USA"},{"name":"Department of Molecular Medicine Zucker School of Medicine at Hofstra\/Northwell  Hempstead New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Vambutas","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Zucker School of Medicine at Hofstra\/Northwell  New Hyde Park New York USA"},{"name":"Feinstein Institute for Medical Research  Manhasset New York USA"},{"name":"Department of Molecular Medicine Zucker School of Medicine at Hofstra\/Northwell  Hempstead New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2004.07.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3233\/VES\u2010150549"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI76503"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537\u2010200311000\u201000008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e3181c34e53"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/mao.0b013e31815dbafc"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201008967\u20107"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.130845"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405\u2010013\u20102596\u20108"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018\u2010200629110\u201000004"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201023911\u20104"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2016.07.030"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2010.08.005"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0000000000003196"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH\u2010190589"}],"container-title":["Laryngoscope Investigative Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lio2.70032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T12:00:57Z","timestamp":1734955257000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lio2.70032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,7]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/lio2.70032"],"URL":"https:\/\/doi.org\/10.1002\/lio2.70032","archive":["Portico"],"relation":{},"ISSN":["2378-8038","2378-8038"],"issn-type":[{"value":"2378-8038","type":"print"},{"value":"2378-8038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,7]]},"assertion":[{"value":"2024-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70032"}}