{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:00:39Z","timestamp":1787954439265,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to examine the effect of topical <jats:styled-content style=\"fixed-case\"><jats:italic>Nigella sativa<\/jats:italic><\/jats:styled-content> oil (<jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content>) on intraoral wound healing in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Forty\u2010eight female Wistar albino rats (2.5\u20133\u2009months old) were assigned to two groups (<jats:italic>n<\/jats:italic>\u2009=\u200924 each): A control group receiving no treatment and an <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content> group receiving a topical application of 1\u2009cc <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content>. A standardized circular full\u2010thickness wound (3\u2009mm in diameter) was created on the mucoperiosteum of the hard palate. The animals were sacrificed on postoperative Days 3, 7, 14, and 20 to assess wound area macroscopically, wound diameter histologically, and immunohistochemically for anti\u2013transforming growth factor\u2010\u03b2 (anti\u2013<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2) and anti\u2010vascular growth factor (anti\u2013<jats:styled-content style=\"fixed-case\">VGF<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant differences were observed between groups on days 3 and 20 (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). On Days 7 and 14, however, the <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content> group exhibited significantly smaller wound areas and wound diameters (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Immunohistochemical staining showed lower <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 expression on Days 7 and 14 in the <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content> group compared to control (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), whereas <jats:styled-content style=\"fixed-case\">VGF<\/jats:styled-content> staining did not differ significantly (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Topical <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content> accelerated the healing of intraoral wounds, particularly by enhancing re\u2010epithelialization between postoperative days 7 and 14. This effect appears to be mediated through <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 modulation and <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content>'s known anti\u2010inflammatory, antimicrobial, and antioxidant actions. Topical <jats:styled-content style=\"fixed-case\">NSO<\/jats:styled-content> may thus be considered as an adjunct to conventional wound management in the oral cavity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.70153","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T08:30:43Z","timestamp":1749025843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Efficacy of Topical <scp><i>Nigella sativa<\/i><\/scp> Oil for Oral Wound Healing in Rats"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5835-1044","authenticated-orcid":false,"given":"Alper","family":"Tabaru","sequence":"first","affiliation":[{"name":"Department of Otolaryngology University of Health Sciences  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8245-4166","authenticated-orcid":false,"given":"Sedat","family":"R\u00fczgar","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ba\u011fc\u0131lar Training and Research Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11081165"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1375784"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina58060835"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405\u2010020\u201005872\u20105"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12663\u2010022\u201001706\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1241660"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07039"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00067.2017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009337"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089475"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00507"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568\u2010015\u20100116\u20105"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.10.029"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221\u20101691(13)60075\u20101"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871529X18666180212114816"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjorl.2022.09.003"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb45110567"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASW.0000418541.31366.a3"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020306"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4MR0316\u2010102R"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13061784"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010023\u201008363\u2010y"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12398"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100722.1996.tb00090.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12065"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e31827a2be1"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7756226"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.35375"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010024\u201004648\u20102"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1432"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058626"},{"issue":"3","key":"e_1_2_10_33_1","first-page":"341","article-title":"Inhibition of Methylnitrosourea (MNU) Induced Oxidative Stress and Carcinogenesis by Orally Administered Bee Honey and Nigella Grains in Sprague Dawely Rats","volume":"21","author":"Mabrouk G. 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