{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:43:39Z","timestamp":1792608219640,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Cisplatin (CDDP) is a potent anticancer agent with significant side effects, including ototoxicity. Capsaicin (CAPS), the active compound in chili peppers, has shown protective effects against CDDP\u2010induced toxicities. This study aimed to investigate the role of capsaicin in CDDP\u2010induced ototoxicity via DNA damage and apoptotic cell death pathways in House Ear Institute Organ of Corti 1 (HEI\u2010OC1) cells and to evaluate its effects on neuroblastoma (KELLY) cells under the same conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HEI\u2010OC1 and KELLY neuroblastoma cells were treated with CDDP (100\u2009\u03bcM), CAPS (5\u2009\u03bcM), and CAPS+CDDP combinations for 24\u2009h. Cell viability was assessed using the WST\u20101 assay, and apoptosis was evaluated by Annexin V\/PI flow cytometry. DNA damage\u2010related gene expressions were analyzed in HEI\u2010OC1 cells using a real\u2010time PCR array. CAPS alone did not alter the viability of HEI\u2010OC1 or KELLY cells. CDDP significantly reduced the viability of both.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CAPS\u2009+\u2009CDDP combination significantly decreased KELLY cell viability, while only slightly affecting HEI\u2010OC1 cells. Both CDDP alone and CAPS\u2009+\u2009CDDP induced similar levels of apoptosis in HEI\u2010OC1 cells. In KELLY cells, the combination induced greater apoptosis than CDDP alone. CDDP treatment upregulated DNA damage\u2010related genes and downregulated DNA repair genes in HEI\u2010OC1 cells. CAPS\u2009+\u2009CDDP treatment altered expression profiles, suggesting a role in DNA repair modulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Capsaicin may serve as a moderate protective agent against cisplatin\u2010induced ototoxicity without compromising cisplatin's cytotoxic effects on neuroblastoma cells. Changes in DNA repair\u2010related gene expression support capsaicin's involvement in modulating the cellular response to cisplatin.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lio2.70598","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:19:24Z","timestamp":1792606764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Capsaicin Modulates\n                    <scp>DNA<\/scp>\n                    Damage Related Gene Expressions in Cisplatin\u2010Induced Ototoxicity"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5632-312X","authenticated-orcid":false,"given":"Yavuz Kerem","family":"Altun","sequence":"first","affiliation":[{"name":"Department of Basic Oncology Dokuz Eyl\u00fcl University Institute of Oncology  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1769-4224","authenticated-orcid":false,"given":"Y\u00fcksel","family":"Olgun","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Dokuz Eyl\u00fcl University Faculty of Medicine  \u0130zmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4307-4878","authenticated-orcid":false,"given":"Ay\u00e7a Pamuko\u011flu","family":"Kaynar","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Dokuz Eyl\u00fcl University Institute of Oncology  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7658-5565","authenticated-orcid":false,"given":"Safiye","family":"Akta\u015f","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology Dokuz Eyl\u00fcl University Institute of Oncology  Izmir 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