{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T04:23:34Z","timestamp":1751775814362},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of this study was to evaluate the in vitro antibacterial effects of a <jats:italic>p\u2010<\/jats:italic>Cymene\u2010based bis(pyrazolyl)methane derivative (SC\u201019) to advance in developing alternative therapeutic compounds to fight against bacterial isolates from patients with otitis externa (OE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen swab specimens were collected from patients aged over 18\u2009years diagnosed with OE within at least 7\u2009days of symptom onset, contaminated by only one bacterium type: <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u20095); <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u20098); <jats:italic>Klebsiella aerogenes<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u20092); <jats:italic>Serratia marcescens<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u20091); <jats:italic>Morganella morganii<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u20092). To appraise antibacterial activity, minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) assays were run at different SC\u201019 concentrations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When using SC\u201019, <jats:italic>S. aureus<\/jats:italic> strains showed less bacterial growth, but no bactericidal effect was observed. The MIC and MBC of SC\u201019 were 62.5 and 2000\u2009\u03bcg\/ml against <jats:italic>S. aureus<\/jats:italic> and were &gt;2000\u2009\u03bcg\/ml against the other isolates obtained from OE, respectively. In addition, the MBICs and MBECs of SC\u201019 against <jats:italic>S. aureus<\/jats:italic> were 125 and &gt;2000\u2009\u03bcg\/ml, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nowadays the acquired antibiotic resistance phenomenon has stimulated research into novel and more efficient therapeutic agents. Hence, we report that, helped by the structural diversity fostered herein by a range of bis(pyrazolyl)methane derivatives, SC\u201019 can be a promising alternative therapeutic option for treating OE caused by <jats:italic>S. aureus<\/jats:italic> given the observed effects on both planktonic state and biofilm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>IV<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.722","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T09:59:26Z","timestamp":1642499966000},"page":"283-290","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A bis(pyrazolyl)methane derivative against clinical <i>Staphylococcus aureus<\/i> strains isolated from otitis externa"],"prefix":"10.1002","volume":"7","author":[{"given":"Ana V.","family":"Oca\u00f1a","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n en 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