{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T21:25:32Z","timestamp":1773264332447,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>The clinical characteristics and prognosis of HPV\u2010related nasopharyngeal cancer (NPC) remain controversial. The relationship between p16 status and outcome was retrospectively investigated in the NPC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Between May 2009 and May 2019, 81 NPC patients who received definitive radiation therapy, in a hospital in Japan, were identified and the prognosis was investigated. p16, p53, and Epstein\u2013Barr virus (EBV) status were assessed. Also, circumferential tumor extent in the nasopharyngeal cavity was assessed on a 5\u2010point scale.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nine and 72 patients were p16\u2010positive and p16\u2010negative, respectively. Fewer patients were EBV\u2010encoded RNA in situ hybridization (EBER\u2010ISH)\u2010positive in the p16\u2010positive group than in the p16\u2010negative group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01). Seventy\u2010five patients were nonkeratinizing NPCs, and six patients were keratinizing NPCs. There were two p16\u2010positive patients among the keratinizing NPCs.<\/jats:p><jats:p>The mean circumferential tumor extent scores of 16\u2010positive and p16\u2010negative NPCs were 4.2 and 3.2, respectively with a statistically significant difference (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.02). Two\u2010year progression\u2010free survival (PFS) of p16\u2010positive and p16\u2010negative patients undergoing chemoradiation therapy were 100% and 69%, respectively (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.13).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study conducted in Japan, p16\u2010positive NPC patients are minor but not very low, and the proportion of keratinizing NPCs was small. p16\u2010positive NPCs were seen both in keratinizing and nonkeratinizing NPCs. P16\u2010positive NPC had a tendency of better PFS than p16\u2010negative NPC. This better prognosis might be due to the higher radiosensitivity of the p16\u2010positive cell. Additionally, p16\u2010positive NPCs seemed to spread more extensively in circumference along the nasopharyngeal mucosa than p16\u2010negative NPCs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.832","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T10:10:27Z","timestamp":1655201427000},"page":"994-1001","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Clinical impact of p16 positivity in nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9094-2637","authenticated-orcid":false,"given":"Yuri","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology National Cancer Center Hospital  Tokyo Japan"},{"name":"Shin\u2010Matsudo Accuracy Radiation Therapy Center Shin\u2010Matsudo Central General Hospital  Chiba 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