{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T14:43:20Z","timestamp":1782398600604,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T00:00:00Z","timestamp":1663977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Compare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross\u2010linked jellyfish collagen \u201ccollagen Type 0\u201d (MX\u2010JC) against two clinical products for injection medialization laryngoplasty (IL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (<jats:italic>N<\/jats:italic>\u00a0=\u20096\/group). Group 1 received (MX\u2010JC) and adipose\u2010derived stem cells (ADSCs), Group 2, MX\u2010JC alone; Group 3, cross\u2010linked hyaluronic acid; and Group 4, micronized acellular dermis. Animals were sacrificed at 4 and 12\u2009weeks. Proteomic profiling of injected versus noninjected VFs by nano\u2010liquid chromatography, tandem mass spectrometry, and reactome gene ontology analysis was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 37\u201361 proteins were found to be upregulated and 60\u2013284 downregulated in injected versus non\u2010injected VFs (&gt;1.5 fold, false discovery rate\u2010adjusted <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Over\u2010representation analysis (% of total) revealed top up\u2010regulated pathways at 4 and 12\u2009weeks, respectively: Group 1, keratan sulfate metabolism (46%) and cellular processes (29%); Group 2, extracellular matrix (ECM)\/collagen processes (33%) and beta oxidation (39%); Group 3, cellular processes (50%) and energy metabolism (100%); and Group 4, keratan sulfate metabolism (31%) and inflammation (50%). Top downregulated pathways were: Group 1, Inflammation (36%) and glucose\/citric acid metabolism (42%); Group 2, cell signaling (38%) and glucose\/citric acid metabolism (35%); Group 3, keratan sulfate metabolism (31%) and ECM\/collagen processes (48%); and Group 4, glucose\/citric acid metabolism (33%) and ECM\/collagen processes (43%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MX\u2010JC \u201ccollagen Type 0\u201d upregulates pathways related to ECM\/collagen formation and downregulates pathways related to inflammation suggesting that it is promising biomaterial for IL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.924","type":"journal-article","created":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T07:40:15Z","timestamp":1664005215000},"page":"1513-1520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Larynx proteomics after jellyfish collagen <scp>IL<\/scp>: Increased <scp>ECM<\/scp>\/collagen and suppressed inflammation"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6542-7932","authenticated-orcid":false,"given":"Andrew J.","family":"Bowen","sequence":"first","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8770-8463","authenticated-orcid":false,"given":"Dale C.","family":"Ekbom","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Hunter","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8010-961X","authenticated-orcid":false,"given":"Stephen","family":"Voss","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Bartemes","sequence":"additional","affiliation":[{"name":"Mayo Clinic ENT  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Mearns\u2010Spragg","sequence":"additional","affiliation":[{"name":"Jellagen Ltd. 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