{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T14:29:06Z","timestamp":1781620146372,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T00:00:00Z","timestamp":1510099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Stig och Ragna Gorthons Foundation","award":["84759"],"award-info":[{"award-number":["84759"]}]},{"name":"Stig och Ragna Gorthons Foundation","award":["86551"],"award-info":[{"award-number":["86551"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>A locally disturbed commensal microbiome might be an etiological factor in chronic rhinosinusitis (CRS) in general and in CRS without nasal polyps (CRSsNP) in particular. Lactic acid bacteria (LAB) have been suggested to restore commensal microbiomes. A honeybee LAB microbiome consisting of various lactobacilli and bifidobacteria have been found potent against CRS pathogens in vitro. Recently, we examined effects of single nasal administrations of this microbiome in healthy subjects and found it inert. In this study, we examined effects of repeated such administrations in patients with CRSsNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>The study was of a randomized, double\u2010blinded, crossover, and sham\u2010controlled design.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty patients received 2 weeks' treatment administered using a nasal spray\u2010device. The subjects were monitored with regard to symptoms (SNOT\u201022 questionnaire, i.e., the primary efficacy variable), changes to their microbiome, and inflammatory products (IL\u20106, IL\u20108, TNF\u2010, IL\u20108,a, and MPO) in nasal lavage fluids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neither symptom scores, microbiological explorations, nor levels of inflammatory products in nasal lavage fluids were affected by LAB (c.f. sham).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two weeks' nasal administration of a honeybee LAB microbiome to patients with CRSsNP is well tolerated but affects neither symptom severity nor the microbiological flora\/local inflammatory activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>1b<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.93","type":"journal-article","created":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T09:22:37Z","timestamp":1510132957000},"page":"410-416","source":"Crossref","is-referenced-by-count":53,"title":["Clinical efficacy of a topical lactic acid bacterial microbiome in chronic rhinosinusitis: A randomized controlled trial"],"prefix":"10.1002","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9262-0001","authenticated-orcid":false,"given":"Anders","family":"M\u00e5rtensson","sequence":"first","affiliation":[{"name":"Department of ORL Sk\u00e5ne University Hospital Lund Sweden"},{"name":"Helsingborg Hospital, Helsingborg, Sweden; the Department of Clinical Sciences Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milad","family":"Abolhalaj","sequence":"additional","affiliation":[{"name":"Department of Immunotechnology Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malin","family":"Lindstedt","sequence":"additional","affiliation":[{"name":"Department of Immunotechnology Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"M\u00e5rtensson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias C.","family":"Olofsson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"V\u00e1squez","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Greiff","sequence":"additional","affiliation":[{"name":"Helsingborg Hospital, Helsingborg, Sweden; the Department of Clinical Sciences Sk\u00e5ne University Hospital Lund Sweden"},{"name":"Lund University, Lund, Sweden; the Department of ORL, Head &amp; Neck Surgery Sk\u00e5ne University Hospital Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Cervin","sequence":"additional","affiliation":[{"name":"Department of ORL, Head &amp; Neck Surgery Royal Brisbane &amp; Women's Hospital, School of Medicine, University of Queensland Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1","article-title":"European Position Paper on Rhinosinusitis and Nasal Polyps 2012","volume":"3","author":"Fokkens WJ","year":"2012","journal-title":"Rhinol Suppl"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-014-0504-y"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11234"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003783"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22398"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2013.04.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21762"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12360"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12345"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-008-9202-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033188"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059600-0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-235"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4486.2009.01995.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01890-10"},{"key":"e_1_2_6_17_1","first-page":"26","article-title":"In vivo demonstration of inflammatory mediator release following nasal challenge with antigen","volume":"128","author":"Naclerio RM","year":"1983","journal-title":"Eur J Respir Dis Suppl"},{"key":"e_1_2_6_18_1","first-page":"597","article-title":"Mediator release after nasal airway challenge with allergen","volume":"128","author":"Naclerio RM","year":"1983","journal-title":"Am Rev Respir Dis"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2008.137414"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174614"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1590-8658(07)60003-6"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-015-0583-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2016.05.004"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004050100363"}],"container-title":["Laryngoscope Investigative Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flio2.93","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lio2.93","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:36:40Z","timestamp":1696012600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lio2.93"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,8]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/lio2.93"],"URL":"https:\/\/doi.org\/10.1002\/lio2.93","archive":["Portico"],"relation":{},"ISSN":["2378-8038","2378-8038"],"issn-type":[{"value":"2378-8038","type":"print"},{"value":"2378-8038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,8]]}}}