{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T03:52:48Z","timestamp":1768794768749,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Laryngoscope Investig Oto"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Laryngeal squamous cell cancer (LSCC) is a highly malignant tumor originating from the respiratory system. Circular RNAs have been reported to be associated with the treatment and prognosis of a variety of cancers, including LSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of circBFAR, miR\u201031\u20105p, and collagen type V alpha 1 chain (COL5A1) in LSCC tissues and cells was detected by quantitative real\u2010time polymerase chain reaction. Cell counting kit 8 and 5\u2010Ethynyl\u20102\u2032\u2010deoxyuridine assays were used to detect cell proliferation. Wound healing assay and transwell assay were used to test cell migration and invasion, respectively. The protein expression in LSCC cells was detected with western blot. The relationships between miR\u201031\u20105p and circBFAR or COL5A1 were identified by dual\u2010luciferase reporter assay, RNA\u2010pull down assay, and immunoprecipitation assay. The effect of circBFAR on tumor growth in vivo was detected by tumor xenograft mice experiment. The protein expression of COL5A1 and KI\u201067 in LSCC tissues was measured by immunohistochemistry assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CircBFAR was increased in LSCC tissues and cells, and was related to advanced clinical stage and overall survival of LSCC patients. The cell viability and proliferation were inhibited by circBFAR knockdown and silencing of circBFAR blocked migration and invasion of LSCC cells. CircBFAR knockdown suppressed cell tube formation, and the protein expression of KI\u201067, matrix metallopeptidase 2 (MMP2), and vascular endothelial growth factor A (VEGFA) in LSCC cells. MiR\u201031\u20105p was the target of circBFAR, and the inhibitory effects of circBFAR deficiency on viability, proliferation, migration, invasion, tube formation and the protein expression of KI\u201067, MMP2, and VEGFA in LSCC cells were rescued by miR\u201031\u20105p downregulation. COL5A1 was negatively regulated by miR\u201031\u20105p, and was boosted in LSCC tissues and cells. COL5A1 overexpression reversed the inhibitory effects of miR\u201031\u20105p on LSCC cells. CircBFAR insufficiency hindered tumor growth in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CircBFAR, miR\u201031\u20105p, and COL5A1 in LSCC progression might provide novel therapeutic targets for LSCC clinical intervention.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lio2.966","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T12:24:00Z","timestamp":1667478240000},"page":"1951-1962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>CircBFAR<\/scp> correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through <scp>miR<\/scp>\u201031\u20105p\/<scp>COL5A1<\/scp> axis"],"prefix":"10.1002","volume":"7","author":[{"given":"Hengcui","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Yibin Hospital of Traditional Chinese Medicine  Yibin Sichuan 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