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However, the lysoPL measurement in cerebrospinal fluid (CSF) has not been validated. Therefore, the present study aimed to validate the lysoPL measurement using CSF samples and to elucidate the possible clinical significance of the lysoPL measurement in CSF. For the validation, we observed a good precision and linearity in a sample with high lysoPL levels. The concentrations of lysoPL changed after incubation but the changes were smaller than those observed for serum samples. Moreover, we observed that the CSF levels of 16:0, 18:0 lysophosphatidylcholine, and 18:0, 18:1, and 20:4 lysophosphatidic acid were significantly higher in subjects with central nervous system invasion caused by hematological malignancies or carcinoma than in subjects with no abnormal CSF test results. In conclusion, an LC\u2013MS\/MS quantification system for lysoPL in CSF might be useful and could be applied to clinical laboratory testing.<\/jats:p>","DOI":"10.1002\/lipd.12175","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T21:34:32Z","timestamp":1561584872000},"page":"487-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Evaluation of Lysophospholipid Measurement in Cerebrospinal Fluid Samples using Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"54","author":[{"given":"Yoshifumi","family":"Morita","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory University of Tokyo Hospital, 7\u20103\u20101 Hongo, Bunkyo\u2010ku, 113\u20108655  Tokyo Japan"},{"name":"Department of Molecular Pathology Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1\u20105\u201045 Yushima, 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